B.Sc. III (Semester VI) (Biotechnology) (CGPA) Examination, 2018
fermentation technology
Day and Date Tuesday, 3-4-2018 Max. Marks 70
Time 10.30 a.m. to 1.00 p.m.
Instructions All questions are compulsory.
Figures to the right indicate full marks.
Draw neat labeled diagrams wherever necessary.
1. Choose the correct alternative and rewrite the sentences again. 14
In the fermentation process prevents vortex formation.
antifoam agents baffles
spargers agitator
ii) Sulfite waste liquor is the byproduct of industry.
paper and pulp sugar
dairy pharma
iii) technique is used for selection of auxotrophic mutants.
Alcohol Acid Base Penicillin
iv) End point determination assay are performed for
growth regulators vitamins
antibiotics enzymes
In continuous culture, chemostat is a self balancing culture
system.
nutrient limiting pH limiting
temperature limiting recovery limiting
vi) Exponential growth in Batch culture may be prolonged by adding
acid to media base to media
fresh nutrient to media salts to media
vii) Crystallization is the best established method for recovery of
ethers alcohols
single cell proteins organic acids
viii) Ethanol is purified using
crystallization distillation
centrifugation solvent extraction
ix) Phenyl acetic acid used as precursor in fermentation.
Penicillin Vitamin B12
Citric acid Acetic acid
Citric acid is produced by
Bacillus subtilis Fusarium moniliforme
Micrococcus glutamicus Aspergillus niger
xi) In the fermentation process Redox potential is controlled by adding
ascorbic acid cysteine
glucose all of these
xii) pH indicators are used in the primary screening of producers.
Alcohol Acid Vitamins Penicillin
xiii) Bacillus thurigiensis is used for production.
organic acid insecticide vitamin antibiotic
xiv) Concentration of growth limiting substance is maintained by in
continuous fermentation.
chemostat turbidostat
pH electrode conductivity electrode
2. Define and explain any seven of the given below. 14
microbial growth
ii) aeration
iii) antifoam agent
iv) precursor
distillation
vi) mutation
vii) submerged fermentation
viii) secondary screening.
3. Answer any two of the following. 10
Explain the batch and anaerobic fermentation.
ii) Explain the methodology used for recovery of vitamin B12 and ethanol.
iii) Explain components and working of typical fermenter.
Explain biological assays with example. 4
4. Answer any two of the following. 14
Explain in detail primary screening of antibiotic, vitamin and organic acid
producers.
ii) Write an essay on amylase production.
iii) Write in brief various methods used for maintenance of microorganisms.
5. Answer any two of the following. 14
Write in detail on penicillin fermentation.
ii) Write an essay on downstream processing.
iii) Give the note on raw materials used for industrial media preparation.