B.Sc. (Part III) (Semester VI) (CGPA) Examination, 2018
Biotechnology
Fermentation Technology
Day and Date Thursday, 1-11-2018 Max. Marks 70
Time 10.30 a. m. to 1.00 p. m.
Instructions All questions are compulsory.
Figures to right indicate full marks.
1. Rewrite the following sentences by choosing the correct alternative given
below
14
Chemically well defined fermentation media is called as
Synthetic media Waste as a raw material
Living media Semi synthetic media
The production of substances in industrial microbiology occurs in the
sequence
fermentation, downstream processing, removal of waste, inoculation
Inoculation, downstream processing, fermentation, removal of waste
Inoculation, fermentation, downstream processing, removal of waste
Removal of waste, inoculation, fermentation, downstream processing
Detection and isolation of industrial important microorganisms from soil is called as
assay primary screening
fermentation none of these
technique is used for selection of auxotrophic mutants.
Alkali Acid Alcohol Penicillin
Enzymes are separated from fermented broth by technique.
centrifugation solvent recovery
filtration none of these

Pilot plant fermentor liters capacity.
100-500 1-2
1,00,000 none of these
Industrial production of citric acid is carried out by using
Bacillus licheniformis E.coli
Saccharomyces cerevisiae Aspergillus niger
End point determination assay are meant for substances.
vitamins amino acids antibiotic enzymes
is used as precursor in Penicillin V production.
Phenyl acetic acid Hydroxy Phenyl acetic acid
Phenoxyacetic acid None of these
10) are added in the fermentor used to avoid foam formation.
Silicon compounds Lard oil
Impeller All of these
11) Solvent extraction is used for recovery of
Amylase Penicillin Alcohol Citric acid
12) Cell population is maintained in steady state in culture.
Batch Continuous Fed batch All of these
13) Fungal culture is maintained by using
Soil Lyophilization
Pasteurization Tyndallization
14) Curries medium is used for large scale production of
Penicillin Ethanol Citric acid Wine
2. Answer any seven of the following 14
Recovery of citric acid from fermentation broth.
Give raw materials used for media formulation.
Give fermentation medium and organism used for production of ethanol.
Give examples for Antifoam agents.
Define inoculums preparation.
Culture collection units.
Crowded plate technique.
Primary screening of acid producers.
Aeration and agitation.
3. Answer any two of the following 10
Write on design and characteristics of an ideal fermentor.
Fermentation economics.
Application of computer in fermentation technology.
Write an essay on citric acid fermentation. 4
4. Answer any two of the following 14
Explain in detail Microbial growth kinetics in continuous culture.
Strain improvement by mutation.
Write an account on different methods of filtration and centrifugation
used for purification of fermented broth.
5. Answer any two of the following 14
Explain biological assays.
Preservation and maintenance of industrial strains.
Give an account on types of Fermentation media.