B.Sc. II (Biotechnology) (Semester IV) Examination, 2018
Animal Tisue Culture (Old CGPA)
Day and Date Monday, 7-5-2018 Max. Marks 70
Time 10.30 a.m. to 1.00 p.m.
Instructions All questions are compulsory.
Figures to the right indicate full marks.
Draw neat and labeled diagrams.
1. Rewrite the following sentences by choosing correct alternatives. 14
was the first cloned cell strain isolated by capillary
cloning.
L929 Hela MCF7 BHK
Hela cell line is derived from cell line.
Stomach cancer Cervical cancer
Lung cancer Blood cancer
Which of the following method used for activation of plastic substrate
UV radiation Heat γ radiation Visible light
cells have finite life span.
Tumor Cancerous Transformed Normal
Plasma clot technique is also known as Technique.
Watch glass Grid Raft Cyclic exposure
In natural media most widely used biological fluid as media is
Plasma clot Serum Coconut milk Clots
is a physical method used to get all the cells in same
phase of growth in culture.
TLC HPLC
Cell size and sedimentation Electrophoresis
Most cell lines grow well at pH
7.1 7.2 7.3 7.4
diluent for concentrates of amino acids and
vitamins.
BSS FBS CBS MS
10) is often added to the cell suspension before viable
counting.
Gram stain Tryphan blue
Crystal violet Fluorescein
11) measurement is probably the best method to use as an
indicator for the number of cells in a solid tissue.
Protein Lipid DNA Carbohydrate
12) A colorimetric assay for viable cells has been developed by using
dye.
CTT GTT MTT None of these
13) In primary culture, cell divide to give same type of cells by
process.
Proliferation Differentiation
Cultivation Initiation
14) involves the exposure of the cell suspension to a high
voltage electrical impulse.
Encapsulation Electroporation
Liposome Protoplast
2. Answer the following (any seven) 14
Write short note on synthetic media.
Describe in brief Laminar air flow.
Explain in brief characteristics of animal cell in culture.
How will you select cell line for culture
Define organ culture.
Define cell synchronization.
Explain maintenance of cell line.
Give brief account on purpose of use of bioreactor for animal cell.
Write a note on cell determination by DNA.
3. Answer the following (any two) 10
Explain analysis of cell cycle.
Explain karyotyping for identification of cell lines.
Describe mechanical methods of cell separation.
Write a note on history of ATC. 4
4. Answer the following (any two) 14
Describe in detail serum containing media.
Explain in detail laboratory design for ATC.
Give details of efficiency and productivity of a culture system.
5. Answer the following (any two) 14
Discuss in detail cell counting and monitoring.
Give details of cold trypsinization.
Give details of different techniques of organ culture.