B.Sc. (Semester IV) Examination, 2018
Biotechnology
Animal Tissue Culture (Old CGPA)
Day and Date Tuesday, 11-12-2018 Total Marks 70
Time 10.30 a.m. to 1.00 p.m.
Instructions All questions are compulsory.
Figures to right indicate full marks.
Draw neat and labeled diagrams.
1. Rewrite the following sentences by choosing correct alternatives. 14
Laminar air flow platform sterilized by using
70% ethanol 100% ethanol
50% ethanol 30% ethanol
described procedure to obtain passaged monolayer culture.
Carrel Dulbecco
Butler Freshney
material is used for treatment of substrate surface.
Albumin Transferin
Collagen Casein
Along with nutritional and hormonal factors factor is also essential for survival of cell in culture.
Insulin Lipids
Proteins Stromal
Isolation of specific cell from tissue for culture is carried out by method.
Disaggregation Heat shock
Centrifugation Radiation
Seat
N
o.
Set
P
P.T.O.
serum is particularly used for more demanding cell lines.
Tiger Foetal Bovine
Horse Goat
serum may also be less likely to metabolize polyamines due to lower level of polyamine oxidase.
Camel Bovine
Horse Goat
Most dispersed cell cultures prefer lower tension.
Hydrogen Nitrogen
Carbon dioxide Oxygen
is usually measured by depression of the freezing point.
Osmolality Apoptosis
Temperature Karyotyping
10) An cell counter is suitable for rapid counting of multiple samples of cells in suspension.
Magnetic Electronic
Manual Parabolic
11) The most sensitive assay used for protein determination is
Biuret assay BCA assay
Lowry assay Nicolson's assay
12) identification can be aided by a banding technique.
Histone Protein
MTT Chromosome
13) A cell line can be identified by use of fluorescent labeled antibody specific for antigen.
Membrane Protein
Lipid Internal
14) Encapsulation of DNA in vesicle is an effective way to improve the efficiency of cellular uptake.
Dextran Liposome
Gel DEAE-Dextran
SLR-SJ 36 *SLRSJ36*
Set P
2. Answer the following (any seven) 14
Write a note on substrate for cell growth.
Write a note on CO2 Incubator.
Explain in brief criteria for subculture.
Give brief account on complete media.
Explain in brief cell counting.
Write a note on cell separation.
Enlist physiological properties of media.
Write a note on type of product in production strategy.
Write a note on cell determination by glucose.
3. Answer the following (any two) 10
Explain analysis of cell cycle.
Give details of warm trypsinization.
Give details of cell synchronization.
Write a note on history of ATC. 4
4. Answer the following (any two). 14
Describe in detail serum free media.
Describe Instruments used in ATC.
Discuss glycoprotein production from mammalian cells.
5. Answer the following (any two). 14
Discuss in detail cell counting and monitoring.
Explain selection and maintenance of cell line.
Explain production of blood clotting factors.