B.Sc. II (Semester IV) (CGPA) Examination, 2018
BIOTECHNOLOGY
Plant Tissue Culture
Day and Date Saturday, 5-5-2018 Max. Marks 70
Time 10.30 a.m. to 1.00 p.m.
N. B. All questions are compulsory.
Figures to the right indicate full marks.
Draw neat labelled diagram wherever necessary.
1. Choose and write a correct answer from given four alternatives 14
Concentration of Myo-inositol required to improve cell growth is
100 mg/lit 50 mg/lit
25 mg/lit 10 mg/lit
Naturally occurring cytokinin hormone in plants is
Indole acetic acid Gibberellins
Zeatin Ethylene
Growth regulator used for induction of embryogenesis of
Gibberellins Abcissic acid
Kinetin Glutamine
The commercial bleach used for sterilization of explant is
Sodium hypochlorite Calcium hypochlorite
Bromine water Mercuric chloride
Brown colour developed by callus due to phenol compounds can be
controlled by
Coconut milk Tomato juice
Activated charcoal Yeast extract
The pH of enzyme solution used for protoplast isolation is
4.7 6.0 3.0 6.0
5.0 6.0 4.0 6.0
cells present in the explant undergo differentiation to different
tissues.
Parenchyma Aerenchyma
Sclerenchyma All of these
The temperature normally required for callus formation is
22°C 28°C 30°C 37°C 28°C 37°C 22°C 30°C
are the cells without cell wall.
Protoplast Cytoplast c Chloroplast Chromoplast
10) The different stages of micropropagation was outlined by
Skoog Murashige White Guha
11) Encapsulation of somatic embryos is done by using
Calcium chloride Calcium hydroxide
Sodium alginate Ferrous chloride
12) The term protoplast was coined by
Klercker Hanstein Morel Martin
13) The convenient and most suitable source of protoplast is
Mesophyll tissue Root tips
Shoot tips Callus
14) High auxin concentration results in
Adventitious root formation Callus formation
Hairy root formation None of these
2. Solve any seven of the following 14
Define totipotency.
Define acclimatization.
Define browning of callus.
Define micropropagation.
Define embryoids.
Define packed cell volume.
Define vermiculite.
Define viability of cell.
Define cybrids.
3. Attempt any two of the following 10
Write about the laboratory setup of plant tissue culture and its scope.
Write about pollen culture with neat labelled diagram.
Write about the factors affecting somatic embryogenesis.
Solve 4
Write about the laboratory and personal safety precautions to be taken in a
tissue culture lab.
4. Attempt any two of the following 14
Write in detail about the different media and its composition used in plant
tissue culture and its significance.
Write in detail about the different sterilization techniques employed in plant
tissue culture.
Write in detail about callus culture and its importance with neat labelled
diagram.
5. Attempt any two of the following 14
Explain in detail the major stages of micropropagation and its application.
Explain in detail about the different protoplast isolation method with neat
labelled diagram.
Explain in detail about the androgenic method of haploid production in
angiosperm plants, with neat labelled diagram.