B.Sc. (Biotechnology) (Part II) (Semester IV) (New CBCS) Examination, 2018
plant tissue culture
Day and Date Monday, 10-12-2018 Total Marks 70
Time 10.30 a.m. to 1.00 p.m.
Instructions All questions are compulsory.
Figures to right indicate full marks.
Draw neat and labelled diagrams wherever necessary.
1. Choose one of the correct alternatives from the following 14
was the first person to culture isolated, fully differentiated cells.
Murashige Cocking Skoog Haberlandt
The temperature of autoclave for sterilization of media is maintained at for 20 min.
37 25 121 4
Culture of 'hairy roots' can be produced by transformation of legume plants with
Agrobacterium tumifesiens Agrobacterium rhizogens
Bacillus thuringensis Escherischia coli
is used for surface sterilization of explant.
Sterile water Sodium hypochlorite
Teepol Bavistin
Highest concentration of ethylene exists at the
Growing tips of plants Leaves
In xylem Ripening stage of fruits
culture technique is used for obtaining clonally propagated plants.
Micropropagation Callus
Anther Protoplast
Somatic embryo encapsulated in an artificial endosperm is known as seed.
Synthetic Natural
Zygotic Parthenocarpic
culture is a type of culture in which cells or cell aggregates are cultured in liquid medium.
Suspension Root Callus Embryo
Heritable differences observed among plants propagated through tissue culture of a single mother plant is known as
Somaclonal generation Somaclonal variation
Somatic hybridization Transformation
10) Cryopreservation is preservation and storage of cells, tissues and organs by immersion into
Culture media Autoclave
Liquid nitrogen Water
11) In plant tissue culture, a morphogenetic response that results in the production of new organ, embryo or whole plant from cultured explant or callus is called as
Recombination Apoptosis
Chlorosis Regeneration
12) Reversal of organized structures into an undifferentiated state is called as
Cytodifferentiation Dedifferentiation
Redifferentiation Nucleation
13) is the length of time plants are exposed to light in an alternating light-dark interval sequence.
Photoperiod Phototropism
Incubation Protoperiod
14) is a class of plant growth hormones which are adenine or urea derivatives and cause cell division, cell differentiation, shoot differentiation etc.
Auxine Cytokinine Cytokine Ethylene
2. Answer the following (any seven) 14
What is sterilization Give the methods for sterilization of glassware.
What is surface disinfection Give an example of sterilant.
Explain the levels of safety.
Differentiate between embryo culture and embryogenesis.
Explain the stages of plant hardening.
What is an endosperm Give the importance of endosperm culture.
Explain the scope of plant tissue culture.
What is a cryoprotectant Give an example of it.
Differentiate between hybrids and cybrids.
3. Answer the following (any two) 10
Discuss micropropagation.
Explain of somatic embryogenesis.
Write an account on suspension culture.
Explain General Plant Tissue Culture Laboratory design. 4
4. Answer the following (any two) 14
Explain in detail about-isolation and culture of protoplast.
Give a detailed account on plant tissue culture media.
Explain in detail-different methods of cryopreservation.
5. Answer the following (any two) 14
Explain in brief-Somaclonal variation.
Give a detailed account on somatic hybridization.
Give a detailed account on callus culture.