B.Sc. II (Biotechnology) (Semester IV) (CGPA) Examination, 2018
molecular biology II
Day and Date Friday, 4-5-2018 Max. Marks 70
Time 10.30 a.m. to 1.00 p.m.
Instructions All questions are compulsory.
Figures to right indicate full marks.
Draw neat and labeled diagrams wherever necessary.
1. Rewrite the following sentences by using correct alternatives 14
is synthesized by RNA polymerase III.
mRNA snoRNA rRNA U6 snRNA
Extrinsic termination of transcription is carried out with help of
in prokaryotes.
Sigma factor Rho factor Pol-α Core enzyme
During mRNA processing 7-methylguanosine cap is formed at
end of mRNA molecule.
only at both and
only at none of these
Shine-Dalgarno sequences is called as
Consensus Promoter
Ribosome binding site None of these
In lac operon, Galactoside permease enzyme is encoded from
gene.
lac lac lac lac
is act as initiator tRNA molecule in prokaryotic translation
process.
tRNAmet tRNAfmet tRNApro tRNAval
enzyme is responsible for polypeptide bond formation in
translation process.
Aminoacyl tRNA synthetase DNA glycosylase
Peptidyl di-sulphide isomerase Peptidyl transfersae
model of tRNA was proposed by Robert Holly.
Clover leaf Fluid mosaic Hairpin loop Double helix
Codons GGG, GGA, GGA and GGU specify same amino acid, this property
of genetic code is called
Non-ambiguous Degeneracy
Overlapping Ambiguous
10) Chaperons plays important role in
protein targeting protein folding
protein synthesis protein degradation
11) In lactose operon is act as inducer.
Glucose Galactose Allolactose Fructose
12) The expression of most genes is also regulated by even more distant DNA
elements called
silencers promoters
operators enhancers
13) Spliceosome is responsible for from pre-mRNA molecule.
glycosylation removal of introns
tailing capping
14) Removal of acetyl groups from the core histones by a family of enzymes
called
histone acetyltransferases histone transacetylases
histone methylases histone deacetylases
2. Answer the following (any 14
What is attenuation
ii) Write a note on Chaperons.
iii) What is General transcription factors
iv) What is rho protein
What are translational drugs
vi) What is anticodons
vii) Write a note on mRNA transport.
viii) Write a note CTD domain of RNA polymerase II.
ix) What are transcriptional activators

3. Answer the following (any 10
Explain structure and regulation of trp operon in bacteria.
ii) Describe termination of transcription in prokaryotes.
iii) Explain alternative splicing mechanisms.
Explain structure of ribosome. 4
4. Answer any two of the following 14
Describe mechanism of signal integration with suitable example.
ii) Explain signal transduction in gene regulation with suitable examples.
iii) Describe process of transcription in eukaryotes.
5. Answer any two of the following 14
Explain post-translational modifications in proteins.
ii) Describe process of mRNA processing in eukaryotes.
iii) Describe process of translation in prokaryotes.