B.Sc. II (Biotechnology) (Semester IV) (CGPA)
Examination, 2018
molecular biology Ii
Day and Date Saturday, 8-12-2018 Total Marks 70
Time 10.30 a.m. to 1.00 p.m.
Instructions All questions are compulsory.
Figures to right indicate full marks.
Draw neat and labelled diagrams wherever necessary.
1. Rewrite the following sentences by using correct alternative. 14
In eukaryotes, mRNA is synthesized by
RNA polymerase II RNA polymerase III
RNA polymerase I Poly polymerase
In eukaryotic transcription process directly binds to TATA box of promoter.
TF-IIH TF-IID
TF-IIE RNA polymerase II
During mRNA processing polyadenylation is carried out at end of mRNA molecule.
Only at Both and
Only at None of these
Shine-Dalgarno sequences are
AGGAGGU AGGUGGU
AGGAGGAU UCCAUCC
In lac operon, β-galactosidase enzyme is encoded from gene.
lac lac
lac lac
Set
P
is not a termination codon.
UAA UGA
AUG UAG
is known as translocase.
eEF1 Release factor
EF-Tu EF-G
of tRNA molecule is called as amino acid attachment site.
Anticodon TΨC CCA site DHU
One of the following is not a characteristic of genetic code
Non-ambiguous Degeneracy
Non-overlapping Ambiguous
10) is responsible for protein degradation of misfolded proteins.
Lysosome Proteosome
Peroxisome RER
11) In trp operon model tryptophan is act as molecule.
Repressor Apo-repressor
Co-repressor Inducer
12) is an activator required for high level transcription of the lac operon.
Catabolite activator protein Glucose
Regulatory protein Galactose
13) is binds to exonic splicing enhancers during removal of introns from pre-mRNA molecule.
SR proteins U2 snRNP
U6 snRNP U2AF
14) Iron-response element provide binding site for
Iron response protein Iron recognition protein
Iron reserve protein Iron regulatory protein
SLR-SJ 34 *SLRSJ34*
Set P
2. Answer the following (any 14
What is abortive transcription
ii) Write a note on fidelity of translation.
iii) What is TFIIH
iv) What are exon shuffling
What is informosomes
vi) What are transcriptional activators
vii) Write a note on mRNA transport.
viii) Write a note anti-termination.
ix) What are introns
3. Answer the following (any 10
Explain mechanism of translation in prokaryotes.
ii) Describe the process of alternative splicing mechanisms.
iii) Describe process of mRNA processing in eukaryotes.

Explain clover leaf model of tRNA. 4
4. Answer any two of the following 14
Explain post-translational modifications in proteins.
ii) Explain signal transduction in gene regulation with suitable examples.
iii) Describe process of transcription in eukaryotes.
5. Answer any two of the following 14
Explain in detail regulation of lac operon in bacteria.
ii) Describe process of transcription in prokaryotes.
iii) Describe signal integration in gene regulation with suitable example.