B.Sc. (Part II) (Semester IV) (New CBCS) Examination, 2018
Biotechnology
Plant Tissue Culture
Day and Date Saturday, 5-5-2018 Max. Marks 70
Time 10.30 a.m. to 1.00 p.m.
Instructions All questions are compulsory.
Figures to right indicate full marks.
Draw neat and labeled diagrams wherever necessary.
1. Choose one of the correct alternatives from the following. 14
had used culture filtrate of the fungus Myrothecium verrucaria and
thereby introduced the concept of enzymatic isolation of plant protoplasts.
Murashige Cocking
Skoog Nisch
The temperature of growth room in Plant Tissue culture is maintained at

37 25 121 4
Thermolabile components of culture media can be sterilized by
autoclaving UV irradiation
filtration heating
is not used for surface sterilization of explant.
Sterile water Sodium hypochlorite
HgCl2 Alcohol
Highest concentration of auxin exist at the
growing tips of plants leaves
in xylem base of any plant organ
culture technique is used for obtaining haploid plants.
Micropropagation Callus
Anther Protoplast
The phenomenon of suppression of growth of an axillary bud in the presence
of the terminal bud on the branch is known as
apical dominance lateral dominance
organogenesis differentiation
is a tissue arising from disorganized proliferation of cells either
in cultures or in nature.
Shoot Root Callus Embryo
A cell or plant with nucleus of one parent and extranuclear genes of another
or both parents, from the fusion of protoplasts is known as
hybrid protoplast
cytoplast cybrid
10) is preservation and storage of cells, tissues and organs by
immersion into liquid nitrogen.
Cryopreservation Autoclaving
Sterilization Culturing
11) Yellowing or whitening of green tissues in plant occurring due to deficiency
of light, magnesium, iron or other factors is called as
necrosis apoptosis
chlorosis aging
12) Reversal of organized structures into an undifferentiated state is called as
cytodifferentiation dedifferentiation
redifferentiation nucleation
13) is the length of time plants are exposed to light in an alternating
light-dark interval sequence.
Photoperiod Phototropism
Incubation Protoperiod
14) Ability of plant cell to form entire plant is known as
Totipotency Pleuripotency
Integrity Continuity
2. Answer the following (any seven). 14
Mention the methods for sterilization of glassware.
What is surface disinfection Give an example of it.
Explain the levels of safety.
Differentiate between organ culture and organogenesis.
How the plant hardening is carried out
What do you mean by synthetic seed
Set P
Give the advantages of cryopreservation.
Define cytodifferentiation.
Draw a neat labeled diagram of general plant tissue culture laboratory
setup.
3. Answer the following (any two). 10
Discuss organogenesis.
Explain different methods of isolation of protoplast.
Write an account on callus culture.
Explain cryopreservations by freezing treatment. 4
4. Answer the following (any two). 14
Explain in detail about-Somatic embryogenesis.
Discuss the anther and pollen culture for haploid production.
Explain in detail the General Plant Tissue Culture Laboratory design and
equipments used for Plant Tissue Culture technique.
5. Answer the following (any two). 14
Explain in brief-Somaclonal variation.
Give a detailed account on organ culture.
Explain various sterilization techniques used in Plant Tissue Culture
laboratory.