B.Sc. II (Semester IV) (Old CGPA) Examination, 2018
Biotechnology
Plant Tissue Culture
Day and Date Monday, 10-12-2018 Total Marks 70
Time 10.30 a.m. to 1.00 p.m.
N.B. All questions are compulsory.

Figures to the right indicate full marks.

Draw neat labeled diagram wherever necessary.
1. Rewrite the following sentences by choosing most correct alternative. 14
Laminar air flow has a number of small blower motors to blow air which pass through a number of filters.
HPLC HP-TLC HEPA NFT
ii) Tissue culture room can be fumigated overnight and sterilized with
Potassium permanganate and formaldehyde
70% ethanol
Steam
Oxygen
iii) vessels have been used in experiments with plant materials sensitive to gaseous build up within the culture vials.
Fluoro carbonate Polycarbonate
Polypropylene Metalic
iv) is a polysaccharide derived from certain algae and commonly used as a gelling agent in tissue culture media.
Sucrose Macronutrients
Micronutrients Agar
Culture of excised anthers, to obtain haploids is termed as culture.
Anther Pollen
Callus haploid
vi) is an asexual plant multiplication starting from a single individual.
Clonal propagation Cultivation
Clonal variation Callus
vii) Preservation and storage of cells, tissues and organs at temperatures around 190°C or by immersion into liquid nitrogen is known as
Preservation Cryopreservation
Attenuation Hardening
viii) is a culture of suspended cells in continuous supply of nutrients by the inflow of fress medium.
Continuous suspension culture Continuous callus culture
Continuous anther culture Continuous micropropagation
ix) A non-sexual developmental process that produces a bipolar embryo with a closed vascular system from somatic tissue of a plant is called
embryo culture Somatic embryogenesis
Somaclonal variation Organogenesis
is a chemical used for induction of protoplast fusion.
Mutagen Fusagen Indugen Osmoticum
xi) is the differentiation of organs from cultured cells or tissue.
Collagenesis Organogenesis
Regeneration Embryogenesis
xii) is one of the macronutrients which is also a component of chlorophyll.
Magnesium Zinc
Copper Boron
SLR-SJ 35 *SLRSJ35*
Set P
xiii) enzyme/s is/are used for isolation of protoplast by enzymatic method.
Celluloses Hemicelluloses
Pectinases All of these
xiv) Acclimatization of micropropagated plants on a large scale is generally carried out in
Polyhouse Refrigerator
Soil Water
2. Solve any seven of the following 14
Explain the roles of media room and growth room in plant tissue culture laboratory.
ii) What is totipotency How is the concept of totipotency important in plant tissue culture
iii) Explain the methods of sterilization by heat.
iv) What is callus How did callus produce by process of differentiation
What is plant tissue culture medium Explain the role of any two components of it.
vi) Explain in brief clonal propagation and clonal variation.
vii) What is an osmoticum Give an example of it.
viii) What is organ culture Name any two methods of it.
ix) What do you mean by hybrid and cybrid
3. Attempt any two of the following 10
Write a note on instruments required in plant tissue culture laboratory
ii) What is micropropagation Explain the stages of it.
iii) What is somatic embryogenesis Explain the method of artificial seed production.
Explain in brief surface sterilization of explant and laboratory
fumigation. 4
Set P
*SLRSJ35* SLR-SJ 35
4. Attempt any two of the following 14
Give a detailed account on anther and pollen culture.
ii) Explain in detail somaclonal variation.
iii) Discuss the methods of protoplast isolation and protoplast fusion.
5. Attempt any two of the following 14
Write an account on different plant tissue culture media and add a note on media composition with significance and preparation.
ii) Write in detail about cryopreservation and its applications.
iii) Explain in detail about the plant regeneration through organogenesis with neat labeled diagram.