B.Sc. Biotechnology (Part II) (Semester III) (CGPA)
Examination, 2018
ANALYTICAL TECHNIQUES
Day and Date Friday, 27-4-2018 Max. Marks 70
Time 2.30 p.m. to 5.00 p.m.
Instructions All questions are compulsory.
Figures to the right side indicate full marks.
Draw neat labeled diagrams wherever necessary.
1. Rewrite the sentence using correct alternative given below 14
In method N terminal of proteins is determined.
Edman degradation Carboxy terminal
Both and None of this
Paper chromatography is type of chromatography.
Liquid-Liquid Liquid-Solid
Gas-Liquid Gas
In Southern Blotting is hybridised.
DNA RNA Protein None
Radioactivity labeled nucleotides can be visualized in situ by
Flow cytometer AAS
UV Spectrometer Autoradiography
The matrix used in ion exchange chromatograpahy is
Sephadex DEAE SDS PAGE
To measure UV radiation is used.
Protein conc. Lipid conc.
Carbohydrate All of the above
The is used as a cell disrupting agent.
Sulphuric acid Lysosome
PEG All of the above
The material extracted from sea weeds used in electrophoresis is
Polyacrylamide Agarose
Resin Buffer solution
The name of the protine staining dye is
Safranine Diphenyle amine
Basic fuchsin Coomasie brilliant blue
10) The membrane used in blotting of DNA is membrane.
Dialysis Nitrocellulose
Polyethylene All of the above
11) The nuclear fraction is sedimented at rpm.
10,000 1,000 8,000 12,000
12) The DNSA, Anthrone and resorcinol method are methods of
estimation.
Lipids Carbohydrates
Proteins All of the above
13) Volume of per unit time is known as flow rate.
Stationary phase Mobile phase
Slurry Sample
14) gives information needed for the synthesis of oligonucleotide.
Macrosequencing Microsequencing
Blotting Blocking
2. Answer any seven of the following 14
Comment on the role of Dialysis in protein purification.
Give the uses of Western blotting technique.
State the principle of HPLC.
Define Proteomics.
Explain DPA method.
Write the principle of 2D Gel electrophoresis.
Explain Saponification value of lipid.
Give the role of Ammonium persulphate.
Explain method of Ultrafiltration.
3. Write the short answers to (any two) 10
Describe the method of Lowry assay.
Write an account on staining of proteins in gels.
Write the principle of mass spectroscopy.
Discuss the importance of support material in Chromatography. 4
4. Write short notes on any two of the following 14
Explain in brief the working of MALDI.
State the principle of Gel permeation and give its applications.
What is the use of stacking gel and resolving gel in SDS PAGE
5. Attempt any two of the following 14
Write the advantages and limitations of estimation methods.
Describe the method of solvent precipitation.
Explain the method of Edman degradation for protein sequencing.