B.Sc. (Biotechnology II) (Semester IV) (New-CBCS)
Examination, 2018
analytical techniques
Day and Date Tuesday, 11-12-2018 Total Marks 70
Time 10.30 a.m. to 1.00 p.m.
Instructions All questions are compulsory.
Figures to right indicate full marks.
Draw neat and labeled diagrams.
1. Rewrite the following sentences by choosing correct alternatives. 14
of cellulose is used in paper electrophoresis.
15% 45%
95% 35%
Purpose of using gel is to Concentrate Proteins.
Starch Separating
Stacking Agarose
Ultrafiltration is a faster purification technique than
Cell Disruption Centrifugation
Immobilization Dialysis
Salting out is the process of of proteins in solution by the addition of large amount of inorganic salt.
Agglutination Coagulation
Dilution Precipitation
Usually in chromatography stationary phase is water.
Paper TLC
Affinity Gel Permeation
Set
P
Chromatography is method for separation of compounds.
Physical Mechanical
Biological Chemical
BCA stands for
Baltimores Citrates Assay Bicinchoninic Acid Assay
Bromide Carrier Assay Bergmans Centrifugation Assay
reduces disulphide linkage in protein structure.
Potassium acetate SDS
Urea β Mercapto ethanol
ESI crates by holding a liquid at high potential difference.
Molecules Compound
Fragments of molecules Ions
10) In technique pH gradient in gel is used for separation.
SDS-PAGE IEF
Sedimentation PAGE
11) In medicine is used primarily to provide an artificial replacement for lost kidney function.
Dialysis Catalysis
Haemolysis Cell Lysis
12) is the most suitable gas to use as a carrier gas in GLC.
Carbon dioxide Oxygen
Helium Methane
13) are inversely proportional to volatility of analyte in GLC.
Concentrations Partition coefficients
V Volume of stationary phase V Volume of mobile phase
14) Migration rate under unit potential gradient is called as of ions.
Mobility Absorptivity Resistivity V Viscosity
SLR-SJ 30 *SLRSJ30*
Set P
2. Answer the following (any seven) 14
Role of homogenizerincell disruption.
Enlist the advantages and limitations of lowry assay.
How will you apply sample in GLC
Principle of Bradford assay.
Column used in column chromatography.
Define blotting.
Brief account on functional genomics.
How will you carry out cell disruption by organic solvents
Write a note on introduction of proteomics.
3. Answer the following (any two) 10
Define chromatography and describe the descending paper chromatographic technique.
Describe basic principle of electrophoresis.
Describe assay used for iodine value.

Discuss limitations of 2-D gel electrophoresis. 4
4. Answer the following (any two) 14
In which electrophoretic technique proteins get separated by using ph gradient.
Give details of carbohydrate estimation by DNSA method.
Explain disc gel electrophoretic technique for protein.
5. Answer the following (any two) 14
Describe Edman degradation for protein sequencing.
Describe chromatographic technique which uses biological interaction between biomolecules for their separation.
Explain DNA blotting technique.