B.Sc. II (Biotechnology) (Semester III) Examination, 2018
ANIMAL TISSUE CULTURE (New CBCS)
Day and Date Friday, 27-4-2018 Max. Marks 70
Time 2.30 p.m. to 5.00 p.m.
Instructions All questions are compulsory.
Figures to right indicate full marks.
Draw neat and labeled diagrams.
1. Rewrite the following sentences by choosing correct alternatives. 14
L929 was the first cloned cell strain isolated by
capillary cloning cancer tissue
epidermis bone marrow
cell line is derived from cervical cancer cell.
HeLa BHK CHO MCF7
cells have infinite life span.
Lymphocytes Stem cells
Transformed Normal
Liquid culture technique is also known as technique.
Watch glass Grid
Raft Cyclic exposure
In natural media most widely used biological fluid as media is
Plasma clot Coconut milk
Serum Clots
Epidermal portion is used to produce
Artificial skin Feeder layer
Liver Blood vessels
γ radiation used for activation of
Glass surface Steel surface
Plastic surface Metal surface
Avian cells can grow at temperature.
Below 28°C 28 to 37°C
37°C 38.5 to 39°C
HEPES buffer is nothing but
4-(2-hydroxyethyl) 1-piperazineethane sulfonic acid
2-hydroxy ethane-piperazine ethyl sulfonic acid
3-hydroxy ethyl-piperazine ethane sulfonic acid
6-hydroxy ethyl-piperazine ethane sulfuric acid
10) Thymidine chemical used to block the cell in to get cell
synchrony.
Interphase Prophase M-phase S-phase
11) Electronic device used for rapid cell counting is nothing but
Coulter counter Heamocytometer
Flow cytometer Osmometer
12) material is used for treatment of substrate surface.
Albumin Transferrin Collagen Casein
13) Rous and Jones started to use in culture media.
Protein Antibiotics Hormones growth factors
14) Increase in LDH activity in cell culture is indication of
Maximum production of NADH
More non viable cells in culture
More healthy cells in culture
Less non viable cells in culture
2. Answer the following (any seven) 14
Describe in brief hormones present in serum containing media.
Describe cell synchronization by physical method.
Write a note on cell determination by glucose.
Define secondary cell culture.
Explain Raft method of organ culture.
Write a short note on BSS.
Write a note on substrate for cell growth.
Write a note on CO2 incubator.
Explain maintenance of cell line.
3. Answer the following (any two) 10
Write a note on history of ATC.
Explain any two methods of cell determination in culture.
Give importance of DNA fingerprinting and isozymes pattern in cell line
identification.
Explain physico-chemical parameter of culture media. 4
4. Answer the following (any two) 14
Describe different methods of organ culture.
Add a note on ATC laboratory design.
Give details of method of chemical disaggregation.
5. Answer the following (any two) 14
Discuss in detail cell counting and monitoring.
Describe mechanical methods of cell separation.
Describe in detail importance of serum in ATC media.