B.Sc. II (Semester III) (CBCS) Examination, 2018
biotechnology
Animal Tissue Culture
Day and Date Tuesday, 4-12-2018 Total Marks 70
Time 2.30 p.m. to 5.00 p.m.
1. Rewrite the sentence using correct alternative given below. 14
is the concentration of CO2 required for culturing animal cells.
2 1 10% 10 15% 15 20%
is the main constituent of culture for animal cell growth.
Glucose and Glutamine Growth factor
Cytokinin Kinetin
The human fibroblast cell is example of
Stable primary cell line Established cell line
Transformed cell line Secondary cell line
pH of initial animal cell culture is controlled by
presence of CO2 bicarbonate buffer
B Bases Acids
carries DNA into the host cell.
mRNA Vector tRNA rRNA
In year carrel designed suitable flask for routine animal cell culture.
1915 1923 1925 1924
Loss of of cells is often indicated by a damaged cell membrane.
Capability Capacity Intensity Viability
The term implies a medium that has all its constituents and supplements added and is sufficient for the use specified.
MS media Complete media
P Protein media Serum free media
A cell line can be identified by use of fluorescent labeled antibody specific for antigen.
Membrane P Protein Lipid Internal
10) The most sensitive assay used for protein determination is
B Biuret assay B BCA assay
Lowry assay Nicolson's assay
11) serum may also be less likely to metabolize polyamines due to lower level of polyamine oxidase.
Camel B Bovine Horse Goat
12) chemical used to block the cell in S phase to get cell synchrony.
EDTA Streptomycin Tetracyclin Thymidine
13) Transfer of cell from primary culture to form secondary culture is known as
Trypsinization Sub culturing
Enzymatic disaggregation Mechanical disaggregation
14) In primary culture, cell divide to give different type of cells by process.
P Proliferation Differentiation Cultivation Initiation
2. Answer the following (any four). 8
Define organ culture.
Define feeder layer.
Define interferon.
Define cell separation.
Define natural media.
B B

Write notes on (any two). 6
What is meant by genetic engineering
Explain centrifugation.
Define primary culture.
3. Answer the following (any two). 8
Explain methods of cell storage.
Write an account on cell types used for animal tissue culture.
Define vaccine and explain viral vaccine.
B B

Answer the following (any one). 6
Write a note on transfer of naked DNA in mammalian cells.
Write detailed account on synthetic media.
Set P
*SLRSJ24* SLR-SJ 24
4. Answer the following (any two). 10
Define serum and its importance.
Explain anchorage dependent cell.
Write account on standard culture condition for animal tissue culture.
Answer the following (any one). 4
Explain cell hybridization.
Write an account on DNA fingerprinting method.
5. Answer the following (any two). 14
Give detailed account on methods involved in analysis of cell cycle.
Explain laboratory design for animal tissue culture.
Define sterilization and techniques used in sterilization of apparatus and media.