B.Sc. (Biotechnology) (Part II) (Semester III) (New CBCS)
Examination, 2018
biophysical instruments
Day and Date Thursday, 26-4-2018 Max. Marks 70
Time 2.30 p.m. to 5.00 p.m.
Instructions All questions are compulsory.
Figures to the right indicate full marks.
Draw neat labeled diagrams wherever necessary.
1. Rewrite the sentence using correct alternative given below. 14
The frequency of visible radiations range from
200 to 400 nm 200 to 300 nm
400 to 800 nm 800 to 1200 nm
The pH indicator phenolphthalein shows the color change from colorless
to pink, as the pH of the solution
increases decreases
remains constant suddenly changes
In electromagnetic wave, the oscillations are to the direction
of propagation of wave and energy.
right angles left angles
parallel perpendicular
The pH meter measures the potential difference between pH glass electrode
and a reference electrode.
zinc cadmium cobalt calomel
The degree of attenuation of a radiant beam incident on particles suspended
in a medium can be measured by
Turbidometry AAS
Nephlometry XRD
In circular dichorism, the differential absorption of light is analyzed.
polarized reflected inhibited deviated
Photomultiplier is generally used in the detector for the spectroscopic
analysis of region electromagnetic spectrum.
UV-Vis AAS IR Near IR
Seat
No. Set P
Set P
SLR-SA 24 *SLRSA24*
Isopycnic centrifugation is a technique used to separate molecules on the
basis of their
Surface tension Conductivity
Redox potential Buoyant density
Density gradient of is used for the density based separation
of DNA molecules.
Calcium chloride Sucrose
Glycerol Agarose
10) In bright field microscopy the object is observed against
background.
Dark Bright Fluorescent Aqueous
11) In centrifugation, for rotation of an object around the fixed central axis, a
strong force to the axis of spin has to be applied.
equatorial parallel
perpendicular longitudinal
12) is the dimensionless number which indicates the propagation
of light through the medium.
Numerical aperture Refractive index
Optical distance Resolution power
13) In GM counter, the Geiger Muller tube is used as a sensing element for
detection of radiation.
mutagenic emerging ionizing scintillating
14) Variations in the optical rotation of a substance with changing light
wavelength are analyzed in
XRD FACS GM counting CD-ORD
2. Answer any seven of the following. 14
Which indicators can be used for pH measurement
What is difference between scanning and transmission electron microscopy
Define radioactivity. State its unit.
State different wavelength ranges of an electromagnetic spectrum.
Give principle of flow cytometry.
Which techniques can be used for detection of radioactivity
Write about relative centrifugal force (RCF).
Draw a neat labeled diagram of image formation in light microscopy.
How calibration of pH electrode can be done
Set P
*SLRSA24* SLR-SA 24
3. Answer any two of the following. 10
Write about nature of radioactivity. Enlist types of radioactive decay.
Describe the isopycnic centrifugation.
Write about molecular energy levels of an electromagnetic spectrum.
Describe different types of rotors used for centrifugation. 4
4. Answer any two of the following. 14
Describe CD-ORD as a molecular characterization technique.
Describe hazardous effects of radioactivity. Add a note on safety measures
for handling of radioisotopes.
Describe construction, working and applications of IR spectroscopy.
5. Answer any two of the following. 14
Write optical principle of microscopy. Describe different types of microscopic
techniques.
Write an account on Nuclear Magnetic Resonance spectroscopy.
Write an account on pH meter. Describe the errors in pH measurement.