B.Sc. II Biotechnology (Semester III) (CBCS) Examination, 2018
biophysical instruments
Day and Date Monday, 3-12-2018 Max. Marks 70
Time 2.30 p.m. to 5.00 p.m.
Instructions All questions carry equal marks.
Figures to right indicate full marks.
Draw neat and labeled diagrams.
1. Rewrite the following sentences by using correct alternative. 14
The first working microscope was designed by
Robert Hook Kepler
Leeuwenhoek Watson
A protein solution shows maximum absorption in range of electromagnetic wavelength.
UV Visible
IR Microwave
The pH electrode can be calibrated by using a standard solution which resists the change in pH.
inorganic organic
salt buffer
Pulses of light generated due to ionization of a material by are detected in
X ray diffraction Flow cytometry
GM counter Scintillation counter
In UV visible spectroscopy, the filament lamp is generally used as a source for generation of UV radiations.
xenon tungsten
deuterium hydrogen
Molecules can be separated based on their buoyant density by using centrifugation.
ion exchange rate zonal
isopycnic two dimensional
Phase contrast microscopy can show the differences in as difference in contrast.
Darkness Brightness
Numerical aperture Refractive index
In circular dichorism, the differential absorption of light is analyzed.
polarized Reflected Inhibited Deviated
In technique, the cells are suspended in a stream of fluid and passed through electronic detection apparatus for detection.
Nephlometry Flow cytometry
NMR AAS
10) The rays emitted by radioactive isotopes have least penetrating power.
alpha beta UV gama
11) The frequency of molecular vibrations range from
105 to 108 Hz 108 to 1011 Hz
1012 to 1014 Hz 106 to 109 Hz
12) The pH meter measures the potential difference between pH glass electrode and a reference electrode.
zinc cadmium cobalt calomel
13) The Geiger Muller tube is used as a sensing element for detection of radiation.
mutagenic emerging ionizing scintillating
14) The pH indicator phenolphthalein shows the color change from colorless to pink, as the pH of the solution.
decreases increases
remains constant suddenly changes
2. Answer any four the following. 8
What is rate zonal centrifugation
What are safety measures while handling radioisotopes
Differentiate between dark field and bright field microscopy.
State different wavelength ranges of electromagnetic spectrum.
Draw a neat labeled diagram of pH meter.
Write a note on any two of the following. 6
Write about sedimentation and RCF. State relationship between them.
Explain principle of fluorescence microscopy. Give examples of fluorescent stains.
Write principle and instrumentation of IR spectroscopy.
3. Answer any two of the following. 8
Describe errors in pH measurement.
Describe instrumentation and application of colorimeter.
Describe the nature of radioactivity.
Descrie in detail any one of the following techniques. 6
Scintillation counter.
X ray differaction.
4. Describe in detail any two of the following. 10
Ultracentrifugation.
Radioactive decay and its types.
Applications and biohazards of radioisotopes.
Describe any one of the following 4
Molecular energy levels.
Types of rotors for centrifugation.
5. Write a detailed account on any two of the following. 14
Electron microscopy and its types.
Principle, instrumentation and applications of UV-Visible spectroscopy.
Principle working and applications of circular dichorism.