B.Sc. II (Biotechnology) (Semester III) (New CBCS) Examination, 2018
BASICS OF MOLECULAR BIOLOGY
Day and Date Wednesday, 25-4-2018 Max. Marks 70
Time 2.30 p.m. to 5.00 p.m.
Instructions All questions are compulsory.
Figures to the right indicate full marks.
Draw neat labelled diagram wherever necessary.
1. Choose and write correct answers from the given four alternatives 14
In Blenders experiment the phage protein was labelled with the help of

32PO4 35SO4 T2 15N
Bent DNA structure can be produced by antitumor drug
Ciprofloxacin Novobiocin Nalidixic acid Cisplastin
Circular DNA found in bacteria and phages biochemically has
bases in more amounts.
A T A G G ≡ C d)G ≡ T
Arthur Korenberg identified enzyme involved in replication.
DNA polymerase III DNA polymerase I
DNA Ligase DNA primase
The number of base pairs of DNA in E.coli origin is
245bp 220 bp 232 bp 241 bp
The length of the DNA replicated in 30 minutes in E.Coli is
10 mm 1 mm 100 mm 1000 mm
The percent of DNA Protein in T2 bacteriophage is
50 50 50 30 40 50 30 50
The SOS repair mechanism is activated by
5-Bromouracil Hydroxylamine
Acridine Orange Thymidine dimers
Set P
Set P
SLR-SA 23
M. Messelson and F. W. Stahl verified semi conservation nature of DNA
replication by using
Autoradiography Fluorescent lable
Electron microscopy Isotopic labelling
10) The mode of replication which resemble the Greek Letter sigma is

Replicon model D-Loop model
Rolling circle model Replication fork model
11) The two helix of DNA are held together by hydrogen bonds in which two
electronegative atoms share between the bases.
Proton Neutron
Electron Positron
12) The codon referred to as Amber is
UAG UGA AUG AUA
13) The protein present in Eukaryotic DNA is
Arginine Lysine Methionine Histone
14) The existence of DNA in the Keto and Enol form is
Isomerism Tautomerism
Phosphorylation Hydroxylation
2. Solve any seven of the following 14
Define Nucleoside.
Define Housekeeping Genes.
Define Leading Strand.
Define Deamination.
Define Cot curves.
Define Wobble hypothesis.
Define DNA damage.
Write whether the DNA is single stranded or double stranded.
A 33% G 17% T 33% C 17%.
Define Solenoid model.
Set P
SLR-SA 23
3. Attempt any two of the following 10
Write in detail Griffith's transformation experiment with neat labelled
diagram.
Write in detail about the DNA structure with neat labelled diagram.
Write in detail about the organisation of DNA in bacteria with neat labelled
diagram.
Explain about the properties of Genetic Code. 4
4. Attempt any two of the following 14
Explain in detail the enzymes involved in DNA replication of Prokaryotes.
Explain Rolling Circle model and fate of displaced Tail with neat labelled
diagram.
Explain Mitochondrial and chloroplast DNA in detail.
5. Attempt any two of the following 14
Write in detail Mismatch and NHEJ repair mechanism.
Explain the process of replication of DNA in Eukaryotes with neat labelled
diagram.
Write in detail about D-loop model of replication in Mitochondria with neat
labelled diagram.