B.Sc. II (Biotechnology) (Semester III) (CBCS) Examination, 2018
Basics of Molecular biology
Day and Date Saturday, 1-12-2018 Max. Marks 70
Time 2.30 p.m. to 5.00 p.m.
Instructions All questions are compulsory.
Figures to the right indicate full marks.
Draw neat labelled diagram wherever necessary.
1. Multiple choice questions 14
Central Dogma was proposed by
T.H. Morgan Gregor Mendel
Francis Crick J. D. Watson
The enzyme which regulates the level of super coiling of DNA molecule is
DNA Ligase DNA Polymerase
Topoisomerase DNA Helicase
Large Cot values indicates presence of sequences
Highly repeated sequences Moderately repeated sequences
Unique sequences All of these
The bases in DNA are joined to the pentose sugar by
Glycosidic Covalent
Ionic Hydrogen
repeats of Telomeres protect free chromosome ends from degradation.
TTAGGGG TTACCCC
TTAAAAA TTAATTT
The relaxed state of DNA is
Covalent closed DsDNA Closed circular DsDNA
Covalent circular Ds DNA Linear DsDNA
is act as initation codon in genetic code of eukaryotes.
AAA AUA
AUG AAG
deamination of Adenine and Cytosine.
Nitric acid Nitric oxide
Nitrous oxide Nitrous acid
The RNA primer is removed by during replication in Eukaryotes.
PCNA RFC
RNaseH RPA Protein
10) Rolling circle mechanism of replication is observed in
E.Coli TMV
T2phage Muphage
11) 1Mb is equal to
1000bp 100 bp
1000kb 100kb
12) The smallest chloroplast genome is found in
Redalgae Pine
Tobacco Tomato
13) The length of DNA replicated in 30 minutes in E coli is
10 mm 1 mm
10.5 mm 105 mm
14) Mitochondrial DNA Mutation leads to the decline of
Glycolysis Photosynthesis
Gluconeogenesis Oxidative phosphorylation
2. Answer the following (any four) 8
What are nucleosome
What is positive super coiling
What is the biological significance of RNA
What are Cot Curves
What are house keeping genes
Write notes on (any two) 6
Solenoid model of chromatin fibre.
Wobble hypothesis.
Conservative type of replication.

3. Answer the following (any 8
Explain clover leaf model of tRNA with neat diagram.
Explain mitochondrial DNA and write its importance.
Write in detail about topoisomerases.
Answer the following (any 6
Discuss the different types of DNA.
Explain SOS repair Mechanism in DNA.
4. Answer the following (any 10
Explain properties of double helix of DNA with neat labelled diagram.
Describe Meselson and Stahls experiment of DNA replication with neat labelled diagram.
Explain the molecular nature of gene.
Answer the following (any 4
Write about the mismatch repair mechanism of DNA with neat labelled diagram.
Write about the process of denaturation of DNA and its importance.
5. Answer the following (any two) 14
Describe the organization of DNA in Eukaryotes with a neat labelled diagram.
Explain the properties of genetic code with suitable example.
Describe D loop model of replication in mitochondria with a neat labelled diagram.