DISTANCE EDUCATION
M.Sc. (Botany) (Specialization Plant Bio technology)
DEGREE EXAMINATION, MAY 2018.
BIO TECHNIQUES IN BOTANY
(2008 Onwards)
Time Three hours Maximum 100 marks
Answer any FIVE questions.
Each question carries 20 marks.
x 20 100)
1. Write an essay on agaroge gel electrophoresis.
Write notes on 2 D Electrophoresis and its
applications.
Write notes on applications of PAGE.
2. Write an essay on principle, mechanism and
applications of HPLC.
Write an essay on Confocal microscopy.
Write notes on MALDI Tof.
3. Write an essay on Gamma and X-ray techniques in
scientific analysis.
Write an essay on Autoradiography.
Write notes on external factors interfering with
decay detection by LSC.
Sub. Code
23
DE-4082
2
Sp 1
4. Write an essay on DNA sequencing.
Write principles, mechanism of RAPD and its
limitations.
Write notes on AFLP.
5. Write an essay on Transcriptome.
Write note on ProtParam and Compute pI/Mw.
Give an outline about Basic Local Alignment Search
Tool.
6. Write an essay on principle, instrumentation and
applications of ultracentrifugation.
Write an essay on tracking plant gene expression
using bioinformatics tools.
Write notes on FPLC.
7. Describe the principle and instrumentation and
applications of GC-MS.
Illustrate SEM.
Write notes on RFLP.
8. Write an essay on Polymerase Chain Reaction and
its applications.
Write an essay on isoelectric focusing and its
applications.
Write notes on HPTLC.