DISTANCE EDUCATION
M.Sc. (Botany) (Specialization Plant Biotechnology)
DEGREE EXAMINATION, MAY 2016.
BIOTECHNIQUES IN BOTANY
(2008 onwards)
Time Three hours Maximum 100 marks
Answer any FIVE questions.
Each question carries 20 marks.
20 100)
1. Write down the methodology of SDS-PAGE and its
applications.
Explain various types of Agarose gel
electrophoresis.
Comment on the differences between agarose gel
electrophoresis and 2D electrophoresis.
2. Comment on sedimentation coefficient.
Explain the principle, instrumentation and
application of SEM.
Write short note on confocal microscopy.
3. Discuss about the applications of HPLC and FPLC.

Describe GCMS.
Write the merits and demerits of SEM and TEM.
4. Write the applications of radioisotopes.
Write short note on autoradiography.
Describe liquid scintillation spectrometry.
Sub. Code
23
DE-3856
2
wk11
5. Describe the western blotting technique.
Differentiate between southern blotting and
northern blotting.
Discuss the principle and working mechanism of
PCR and its applications.
6. Describe the procedure of RAPD.
Write short note on the application of DNA
sequencing.
Briefly describe the application of DNA
fingerprinting.
7. Write short note on transcriptome and proteomes of
plants.
How will you analyse the plant genomes?
What is the relation between genomes,
transcriptomes and proteomes of plants?
8. Explain about the DNA microarray and NCBI.
Describe the tracking gene expression in plant cells.

Distinguish between genomes and proteomes.