Master of Science II (Microbiology) Examination: Oct Nov 2016
Semester II (New CBCS)
SLR No. Day
Date Time Subject Name Paper
No. Seat No.
SLR S0
620
Tuesday
22/11/2016
10.30 AM
To
01.00 PM
Biophysics
Bioinstrumentation
C
VII
Instructions: Part I. Q.1 is compulsory
Attempt any 4 questions from part II
Figures to the right indicated full marks
Answer to the part I and part II are to be written in same answer
booklet only.
Total Marks: 70
PART 1
Q.1 Rewrite the sentences after choosing correct answers from the given alternative. 14
In UV-Visible spectrophotometer, Transmittance I0
90 80
102 100
In Mass Spectroscopy detectors are used
Photomultiplier tube photographic films X-ray Films
Electron multiplier Dielectric multiplier
For visible region is used for reference clamber.
Pyrex glass Quartz
Class of pure silicon dioxide None
The isotopes of an element have the same number of but different
number of
proton, electron proton, neutrons
neutrons, electron only proton
In spectroscopy, the visible regions of the spectrum extend between
colour.
violet, red violet, green
orange, violet red, blue
In most of the Atomic Absorption Spectrophotometer dispersive granting that
have resolution in the range of is used
0.4-0.04 nm 0.2-0.02nm
0.2-0.3nm 0.4-0.5nm
technique is useful for preliminary examination of explosive
materials such as nitrobenzene, TNT, RDX, HMX, Tetryl, etc
UV-Visible Spectrophotometer Radio isotopic technique
Atomic Absorption
Spectroscopy
Atomic emission
Spectroscopy
Page 1 of 2
technique was first carried out for quantification of Insulin in
diabetic patient.
IR RIA
ELISA NMR
X-rays was discovered by in 1895
Rontgen Neubaur
Henry Bequerel None
10) is a versatile, non-destructive analytical technique for
identification and quantitative determination of the various crystalline forms.
Circular Dichroism Optical Rotary Dispersion
Spectroscopy
Nuclear Magnetic Resonance X-ray diffraction
11) is defined as a piece of solid matter in which the atoms are
regularly arranged in space.
Crystal Phase
Crystalloids None
12) In X-ray spectroscopy crystallites give rise to sharp peaks.
Small Large
Very small Very large
13) The 'Fingerprint Region' is related to spectroscopy
Infra-Red X-ray
Raman Nuclear magnetic
14) technique is useful to determine the molecular weight at the
expense of negligible amount of sample.
Mass spectroscopy IR spectroscopy
Raman Spectroscopy X-ray diffraction
PART II
Q.2 Write an essay on "Autoradiography" 14
Q.3 Write in detail about "Nuclear Magnetic Resonance" 14
Q.4 Describe in detail the technique and applications of atomic absorption spectroscopy 14
Q.5 Write a short answers (any two) 14
Discuss briefly the "Raman Spectroscopy"
Give brief account of "Potentiometry".
Describe in brief "voltametry"
Q.6 Write short answers (any two) 14
Discuss briefly "Immunodiffusion"
Briefly describe "Nanometry"
Describe in detail "General principle and applications of electromagnetic
radiation"