1. Which of the following enzymes is
absent in anaerobes?
Catalase
Cytochrome peroxidase
Pyruvate carboxylazc
All of the above
2. Lysogenic conversion refers to
Lysis of bacteria
inclusion of prophage of DNA in
bacteria
Conversion of one bacterial strain
to other
Any of the above
3. IL-2 is secreted by •
CD4 and lymphocytes
CD8 cells
Macrophages
Neutrophils
4. Adenosine deaminase (enzyme)
deficiency is associated with:
Severe Combined lmmunodefi­
ciency Disease (SCID)
X-linked agammaglobulinemia
Transient hypogammaglobull­
nemia of infancy
Chronic granulomatous disease

5. Which molecule serves to destabiliLC the DNA helix in order to open it up, creating a replicating fork?

SSBPs


DNA Ligase


DNA helicase


DNA gyrase



6. The most unsaturated fatty acid among the following is

Arachidonic acid


Oleic acid


Linoleic acid
Linolenic acid

7. In vivo synthesis of DNA is:
to
to

Either way, depending upon the direction of Polymerase


In Leading strand to whereas,


in Lagging Strand to

8. Mad Cow Disease which affiictcd British cows is caused by
Prions

a virus


DDT


Mycoplasma

ST-10IBIOTECHJD 1 P. T.O.

9. Archaebacteria are:

all bacteria which g"row in extreme environmental conditions


prokaryotes, but not bacteria, which are usually found growing under extreme environmental conditions


prokaryotes which are precursors of eukaryotic organelles


fossilized bacteria which mayor may not be revived


10. Which one of the following is not an organelle?

Microsome


Nucleus


Lysosome


Peroxisome


11. Which of the following is a property of eukaryotic DNA?
(aj All DNA encodes protein

Dilferent region of DNA are transcribed in different cell types


DNA is kept from wrapping up into compact structurc by histones


The amount of DNA is proportional to the complexity of the organism


12. Why is RNA primer considcfC.'d essential during DNA synthesis by DNA polymerase I

The enzyme requires a free group


The enzyme requires a free group


The enzymes requires a frce group


The enzyme requires a free group



13. All of the following arc used for isoelectric focusing, except:

a polyacrylamide gel


a pH gradient


an electric charge


SOS



14. Mode of action of Puromycin is:

It acts as a protein-protein binding inhibitor


It binds to tubulin and prevents fonnation of microtubules


It acts as chain terminator in protein synthesis


All of the above



15. Single stranded DNA is not found in:

M-13


Gemini virus


Rous sarcoma virus


0X174

ST-10IBIOTECHJD

16. Bt transgenic cotton with pest resistance
developed with a gene from

Pests


Fungi


Hom worm Larva
Bacteria
17. Which of the foUowing is not a feature of eukaryotic gene expression?
polycistronic mRNAs are very rare
many genes are interrupted by
non-coding DNA sequences


RNA synthesis and protein synthesis are coupled as in prokaryotes


mRNA is often extensively modified before translation


18. In the Meselson-Stahl DNA replication experiment, what percent of the DNA was composed of one light strand and one heavy strand after one generation of growth in 14N containing growth media?
0
25
50
100
19. For the DNA strand 5'-TACGATCATAT-3' the correct complementary DNA strand is

3'-TACGATCATAT-5'


3'-ATGCTAGTATA-5'


3'·AUGCUAGUAUA·5'


3'-GCATATACGCG-5'


20. The Electron Microscope was invented
by:
Knoll and Ruska

Zemike and Zacharius


Koch and Lister


Roentgen and Curie



21. The Golgi apparatus is involved in the synthesis of

Ribosomes


Lysosomes


Mesosomes


Nucleosomes

22. Blood group with antigen and antibody is
A
AB
B
0

23, Which of the following is not required when recombination is considered at molecular level in E. coli?
A nicking of sugar phosphate backbone
Strand displacement
Ligation of sugar phosphate backbone
RNA synthesis

ST-101BIOTECHJD P. T.O.

24. Pectin is a polymer of: 29. The characteristic clover leaf model of tRNA is attributed to its
Pectic acid

Priny.ary structure


Galacturonic acid



Secondary structure
(cJ Culuronic acid



Tertiary structure

Glucuronic add Quaternary structure
25. Which one is not tnte of Chargaffs 30. Bacterial metabolism in which the cell
law? oxidises inorganic compounds to produce organic molecules from CCh
A=TandG=C in the absence of light is called
Cherno autotrophy



Chemo heterotrophy
(el Photo autotrohphy

Photo heterotrophy

31. The protein moiety of an cm'.ymc is26. The tcnnination codon of polypeptide
known as:
synthesis is

(aJ UAA UAG Holoenzyme
UGA All of the above Apoenzyme
Coenzyme
27. The bacterial cell wall component susceptible to Lysozyme is Isoenzyme
28. Lipopolysaccharide Teichoic acid Peptidoglycan Lipoprotein Which of the following occur in mitochondria? ATP synthesis processes 32. Nuclcotidcs are composed of Nitrogenous base and pcntosc sugar Nitrogenous base, pcntosc sugar and phosphate group Nitrogenous base and two phosphate groups
DNA synthesis Protein synthesis Nitrogenous base phosphate groups and three
All of the above

33. One complete Kreb's cycle starting
from oxaloacetate produces how many
moles of ATP
3
12
34. The main source of cholesterol from
the following is
Animal fats
Vegetable fats
Egg yolk
AU the three
35. Amino acids in solution at neutral pH
are predominantly:
Cations
Anions
Zwitterions
None of the three
36. Which of the following is 'not an
essential amino acid?
Leucine Valine
Threonine Alanine
37. The net gain of ATP per molecule of
glucose metabolized aerobically in
prokaryotes is
2 32
38 85

38. Which one of the following statements is incorrect

Lactate produces D-lactic acid.


Ethanol production by yeast involves pyruvate decarboxylase enzyme.


Acety eoA is not an intermediate in the formation of ethanol by yeast.


None of the above



39. Which of the following statements is correct

Polycistronic mRNA produces a large protein which gets broken into individual proteins,


Polycistronic mRNA contains multiple si'tes for the assembly of ribosomes.


Polycistronic mRNA gets broken into fragments which upon translation results in the formation of individual proteins.


Ribosomes assembled at the region of the polycistronic mRNA continue to· moves along to produce individual proteins.



40.
Structurally asynunetric lipids with polar and non-polar ends are said to
be:

Amphipathie


Amphibolic


Bilateral


None ofthese



ST-1OIBIOTECHJD P. T.O.

•


41. Which of the following 15 not characteristic of prokaryotic promoters
They bind RNA polymerase

They are dose to the transcription start site


They have an A-T rich Pribnow­


box
They have a pyrimidine-rich

Shine-Dalgamo box

42. Proteosomes are:

Cellular organelles involved in protein trafficking


Macromolecules that autodigest mitochondria


Ribonucleoprotein complex repaired for protein synthesis


Cd) Macromolecules that degrade ubiquinated proteins

43. A radioisotope with the longest half­
life is:
14Carbon

'Tritium


"Phosphorus


12s1odine



44. In signal transduction, scaffold proteins

pass the message to the next signaling component


convert the signal to a different


form


bind multiple signaIing proteins in a functional complex


greatly increase the signal they


receive

45. The length of the 'leader peptide' associated with attenuation of tryptophan operon is
1410 amino acids

141 amino acids


41 amino acids


Cd} 14 amino acids

46. DNA fragments larger than 100 I«b can be more effectively resolved by:
Agarose gel clectrophorsis

50s-PAGE


Sequencing Gel


Pulse Field Gel electrophoresis

47. Void volume in gel filtration is:
Total volume in the beads

Volume of buffer between thc beads


Both


One fourth of



48. In differential centrifugation, mitochondria sediment between:
200 x g to 1,000 x g

500xgto10,000xg


10,000 x g to 20,000 x g


20,000 x g to 1,00.000 x g



49. Regulation of gene expression by attenuation was discovered in
lac operon

Arabinose operon


5hikimate pathway


trp operon



ST-101BIOTECHJD (6



50. In cloning experiments, sell-ligation of vector can be avoided by
End filling by Klenow
Phosphatasing the ends

Polishing the ends with 51
nuclease
any of the above

51. Enhancer elements are found in

coding region


upstream of coding region


down stream of coding region


Any of the above region

52. A reagent which will increase the Tm of a DNA solution is
NaCl

Urea


Methanol


AU of the above



53. Central Dogma of Molecular Biology does not includes
(aJ DNA to DNA

RNA to Protein


DNA to RNA


Protein to RNA



54. Shuttle vectors differ from typical plasmid cloning vectors in having:

a multiple cloning site


more than one antibiotic resistance gene


more than one centromere sequence


two different origins of replication

55. Among the following, the weakest bond is:

Hydrogen bond


Ionic bond


Covalent bond


Vander Waol's bond



56. The number of sense codons in the "standard" genetic code is
20

44


61


64



57. Griffith's experiments with
S. pneumoniae indicated that:
bacteria could exchange genetic information by conjugation
hereditary information could be
stably transferred from one bacterial strain to another
the . hereditary material was unlikely to be made up of protein
the hereditary malerial must be DNA

ST-1 OIBIOTECH./D P. T.

58. An organism which lacks spliceosomes is

Neurospora CTaS$a


Saccharomyces cerevisiae


Bacillus subtilis


all of the above
59. Bird flu·is caused by:

H5NI


HlV



RHINO Virus


REO Virus


60. The relation'>hip between proto­oncogenes and tumor suppressor genes is the following:

Each can lead to malignant transformation when mutated


Both are normal viral genes


Proto-oncogenes are growth promoting whereas tumor suppressor genes are growth retarding


Mutations in tumor suppressor genes are almost always domimmt, while those in proto­oncogenes are generally recessive




61. The excision repair process may begin by recognition of damaged nucleotide or by recognition of damaged basco "Ihe damaged often a cOn5<.."quence of chemical mutagen are recognised by variety ofcell enzyme called

Photolyase Clycosylascs


Ligases Polymerase



62. "Propeller twist" refers to the angle:

of rotation about the helical i'lxis between neighbouring bases on one strand of double-helical DNA


between the plane of a base and that of the sugar to which it is linked


by which a base is tilted away from the plane normal to the helical axis


between the planes of two bases in a base pair



63. Study involving fusion of cells from individuals with Xeroderma pigmentosum have identified 7 different genes that can·cause disease. A patient comes to you with apparent case of Xeroderma pigmentosum. You decide to fuse cells of this patient with the existing cell lines derived from other patients. You discover that when you fuse the cell from patient with cells of patient the hybrid fusion product does not repair the UV lesion. If patient X is deficient in the protein that binds Thymine dimmer, XPA, what do you conclude?

Your patient is also deficient ·in this gene product


Your patient is deficient m different aspect of repair mechanism


Your patient has new gene


None of the above




64. In the beginning of each cycle the temperature of PCR reaction is raised to:

denature the DNA


anneal primers


elongate the product


Ugate·the primer




65. The restriction enzyme Ddel recognizes a DNA sequence, CfCAG, and cut the sequence' at this point. A domain of normal haemoglobin gene contain CCTGAGGAG sequence. A allele responsible for sickle cell anemia is CCTGTGGAG. Which allele will Ddel recognizes and cut?

Normal p-ehain allele


Sickle p-chain allele


Both nonnal sickle chain-allele


Normal a-chain allele



66. DNA fingerprinting using variable number of tandem repeat (VNTRs) is based on the observation that:

VNTRs sequence show little variability


Every individual has unique alleles at each VNfRs locus


VNTR loci are highly polymorphic


DNA of VNTR loci are more stable than that of loci which code for proteins



67, Meat tendering enzyme is
Ca) Papain

Pepsin


TrypSin


Chymotrypsin



68. An organism which does tlot have intracellUlar membrane-bound organelles is

Saccharomyces cerevisiae


Escherichia coli


Plasmodium vivax


gracilis



69. Which of the following is shared by both prokaryotes and eukaryotes

poly A tail


Promoter


lntrons


Splicing



70. Which is tlot a DNA binding domain

zink finger


leucine zipper


intron-exon-intron


heUx tum helix



71. Which activity of DNA Polymerase remove the RNA primer?

to exonuclease activity


to endonuclease activity


to exonuclease activity


Primase activity




ST-1OIBIOTECHJD P. T.O.

72. Which of the following does not explain that how human cell can apparently replicate DNA much faster than bacterium

There arc more ongm of replication in Eukaryotes


The eukaryotic DNA polymerase moves faster than Bacterium


There are more DNA polymerase


in Eukaryotes

DNA synthesis occurs at multiple
points along the chromosomes in
Eukaryotes

73. Wltranslated. regions are present:
only in mRNAs of prokaryotic
genes


only in mRNAs of eukaryotie genes


in mRNAs of both prokaryotic


and eukaryotic genes

only in mRNAs that undergo
splicing

74. DNA was shown by Hershey and Chase to be the genetic material of bacteriophages" by labelling it with:
32p
3H

3SS


ISN


75. A mutation is
a change in nucleotide sequence resulting in a gene product with altered sequence
a change in nucleotide sequence resulting in a gene product with altered function
a change in nucleotide sequence resulting in a phenotypic change only in the homozygous state
any change sequence m nucleotide
76. 'Genetic polymorphism' refers to •
the generation of different forms of a protein encoded by the single gene, by mechanisms such as altemanc splicing
the existence in a population of multiple alleles of a gene
the multiple mutant phenotypes resulting from a mutation in a single gene
the variety of mutant phenotypes of different severity caused by a mutant allele when placed In different genetic backgroWlds
n. An example of a cis-regulatory clement would be an:
inducer
enhancer
transcription factor
aU of the above

ST-1 OIBIOTECHJD

78. A recessive mutant allele of a gene:

is repressed in the presence of the wild type allele


has a more deleterious phenotype than a dominant mutant allele


shows a mutant phenotype only in the homozygous state


All of th.e above



79. The process by which amino acid is covalently bonded to RNA molecule is called

Charging


Translation


Initiation


Wobbling



80. lsoaccepting tRNA molecule are

a subset of tRNA molecule that

I are recognized by particulate aminoacyl tRNA synthetase


an enzyme that causes bonding of amino acid to tRNA molecule


ribosomal initiation factor


enzyme that catalyse the fonnation of peptide bonds



81. Sugars differing in configuration at a single asymmetric centre are

Stereoisomers


Epimers


Enantiomers


Anomers



82. Lectins contain binding sites for:
carbohydrates
lipids
DNA
RNA
83. Average bond enthalpy of bond (kJ mol-I) is
393
460
347
619
84. In bacteria,. just before the cell divides. the two daughter genomes are attached to the:
Cell membrane Replication origin •
Kinetochore
Centromeres
85. cdK is an important protein for the regulation of cell cycle. It is a protein that:
is degraded after it is used
is phosphorylated active to become
binds to different cyctins
manufactures growth factors
86. Which of the following is not a tumour suppressor gene?
APC
NFl
RBI
RET

ST-101BIOTECHJD 11 P. T.O.

87. Breast cancer is associated with:
(al HNPCC
BRCA 2
NFl
REf

B8. Which one of the following is not a second messenger?

cyclic AMP


lnositollriphosphate


Calmodulin


Diacyl gLyceroL



89. "Pruteen" term was given to the single cell protein manufactured by continuous batch culture of
FuSarium graminearum
Methylophilus methylotrophus
Saccharomyces cerevisae
Spirulina Plexu""a

90. Monoclonal.antibodies are associated with the name of

Jenner and J"lin


Emil von Behring


Georges Kohler and Cesar Milstein
Pasteur and Koch

91. Thylakoids are found in

mitochondria
chloroplasts
lysosome.
92. In Pinocytbsis
large. insoluble complexes
penetrate across the plasma
membrane
all soluble molecules penetrate
across the plasma membrane
molecules penetrate with the
assistance of facilitated transport
soluble molecules enter cells in
vesicles that were pinched off
from the plasma membrane
•
93. The Na-K pump moves and
across the plasma membrane by:
facilitated transport
active.transport
ce>-transport
endocytosis
94. The pKa of lactic acid shall have the
following value, given that when the
concentration of lactic add is O.OlOM
and the concentration of lactate
0.087 M., the pH is 4.80
5.06
3.86
4.8
0.54

8"1:-101BIOTECHJD (12

95. A frameshift mutation could be caused
bya:

transition


transversion


insertion


any olthe above.



96. Which of the foods listed would be
most likely to spoil as a resuH of bacterial growth
a meat product with near neutral
pH


fruit in a high sugar syrup



a vegetable in a high salt, acid


brine (liquid)
all of the above

97, Which of the following is best to sterilized heat labile solution?

dry heat


autoclave


membrane filtration


pasteurization



98. The constant k 1.381 X to-D j/K refers to:

Gas Constant


Faraday's Constant


Boltzmann Constant


Planck's Const""t



99. The pore size of a gel is by:
Amounts of acrylamide and cross linker present
Amount of acrylamide presenl
Amount present of mcrcaptoethanol
Amount of TEMED prescnt
100. The cross linking agcnt in SDS-PAGF is:
TEMED
AI'S
SDS
Bis acrylamide
101. The diploid human genome comprises 6.4 x 109 base pairs. 'Ibe length of DNA in a human cell shall be
2.18m
34 em 64m 28 em
102. If a particular reaction has a negative 6G, is it likely to occur? Not unless energy is added to the system Yesr if it is coupled to another reaction
Yes, it can occur spontaneously No, it will not occur

ST-l01BIOTECHJD 13 P. T.O.
103. The Bam lil recognition site which is 6
nucleotide pairs will occur in the
human DNA on an average, once in
20,000 nucleotides
256 nucleotides
4096 nucleotides
5896 nucleotides
104. HIV enter in its host cell via
mediated endocytosis, which of the
following is receptor protein
P-32
GP-120
CD-4
P-18
105. National Gene Bank for Medicinal and
Aromatic Plants has been established
at:
NBPeR, New Delhi
CIMAP, Lucknow
TBGRI, Trivendrum
All of the above
106. Yeast Tyl element is an example of:
a LINE element
a SINE element
a Retrotransposon
None of the above

107. Non·scnse cadons genetic code are: UGA and UGG UAG and UAA AGU and UGU AGA and AGG 10 mitochondrial
108. Confirmatory test for AIDS is: Western Blotting Eastern Blotting Southern Blotting Northern Blotting
109. The enzyme fumarase catalY7..c5 the reversible hydration of fumaric acid to I-malate but it wiU not cataly:t.c the hydration of maleic acid, the cis­isomer of fumaric acid. lhis is an example of: recemization chiral activity isomerisation stereospecificity •
110. On a Ramachandran plot, the degree of rotation between nitrogen and a carbon atoms of the main chain is called psi phi sigma delta

ST-101BIOTECHJD 14)

111. Which of the following rRNAs is
absent in E.coli
235
185
165
55
112. A temperate bacteriophage is
A phage that infects at cold
temperatures
A single-stranded bacteriophage
Remains in integrated and free
forms
A virus that infects bacteria of
temperate animals
113. factor of E.co1i is transferred during
conjugation as a
Single-stranded DNA
Linear double-stranded D A
Circular double-stranded DNA
Concatamer
114. In the crown gall disease caused by
Agrobacterill1n tumefaciens, opines are:
Responsible for tumour formation
Made by Agrobaderium and used
for pathogenesis
Made by tumours and used for
tumour growth
Made by tumours and secreted

115. Which division of bacteria has a gram­positive cell wall

Gracilicutes


Archaea


Firmicutes
Tenericutes

116. The protozoan trophozoite is the:

Active feeding stage


lnactive dormant


Infective stage


Spore forming stage

117. The temperature-pressure combination for an autoclave is

100"C and 4 psi


131"C and 9 psi


121'C and 15 psi


115'C and 3 psi



118. Drugs that prevent the formation of the bacterial cell wall are
Quinolones
Beta-lactams
Tetracyclines
Aminoglycosides

119. Xeroderma pigmentosum is caused
by:

UV radiations


IR radiations


X-rays


p-rays


120. Biotechnological work and its progress in India is looked after by which department under the Ministry Science and Technology? DST DBT MAB lllWL
• •
•

ST-1111BIOTECHJD


(For Rough Work)

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