5/50/2013/12

MICROBIOLOGY


Roll No.


I

BOOKLET NO.

Candidate should write his/her Roll No the box above. i
Time: 2 Hours] I No. of Printed Pages 32 I [Total Marks 300

INSTRUCTIONS FOR CANDIDATES
1.
All questions are compulsory.

2.
All questions carry equal marks.

3.
The question paper contains 150 questions. The examinee should verify that the requisite number of questions are printed in the question paper, otherwise he should ask for another question paper.

4.
The cover page indicates the number of printed pages in the question paper. The examinee should verify that the requisite number of pages are attached in the question paper otherwise he should ask for another question paper.

5.
Read carefully the instructions given on the answer-sheet supplied and indicate your answers accordingly.

6.
Kindly make necessary entries on the answer-sheet only at the places indicated and nowhere else.

7.
Examinees should specially pay attention that 2 marks will be awarded for correct answer.

8.
Examinees should do all rough work on the space meant for rough work on the last page of the question paper and nowhere else, not even on the answer-sheet.


5/50/2013/12

1.
Which of the following is not a cellular microbe

(Al Mycoplasma (Bl E.coli (Dl T4 Bacteriophage

2.
Out of the following, who constructed the Primary Microscope


(Al Leeuwenhoek (Bl Louis Pasteur (Cl Robert Koch 0. Brefeld
3. Role of microbes m N2 fixation was (Al Winogradsky and Beijerinck established' by
(Bl Pasteur and Zenner
(Cl Koch and Iwanowsky
None of the above
Microorganisms known as usmg light as energy and CO2 as carbon source are
(Al Chemoautotrophs (El Photoautotrophs
(Cl Photoheterotrophs (Dl 8/80/2013/12 Chemoheterotrophs

5. Bacterial growth is measured in terms of

Cell mass Cell number


Cell constituent All of these


6. In generalized growth curve maximum growth of bacteria is observed in

Lag phase Exponential phase


Stationary phase None of these


7. Which of the following bacteria IS among the extreme halophiles

Staphylococcus aureus


Micrococcus helodenitrificans
(Cl Halobacterium salinarium
ID) Salmonella oranienburg



8. The capsomere III a VIrus IS made up of
(Ai Fat Carbohydrate
DNA Protein
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9.
Tobacco mosaic virus was first obtained in crystalline form by

(Al D. Iwanowsky (Bl F. Twort (Cl W. Stanley M. Beijerinck

10.
What type of nucleic acid is found in T4 Bacteriophage? (Al Linear dsDNA (Bl Circular ssDNA

Circular dsDNA CD) Linear dsRNA

11.
Aflatoxin is produced by

Bacteria (Cl Virus Fungi

12.
The causal organism of the chickenpox is a

Rickettsia Chlamydia (Cl Mycobacteria Virus

13.
Which of the following is konwn as Brewer's friend

14.
In the root nodules of pulses the common bacterial symbiont is

(Al Agrobacterium (Bl Rhizobium (Cl Clostridium Azotobacter

15.
Bergey's manual of systematic Bacteriology is written in


(Al Saccharomyces (Bl Lactobacillus
Rhizopus None of these
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CAJ 2 volumes (Bl 6 volumes (Cl 4 volumes 3 volumes
16. Cell wall of eubacteria IS made up of
(Al Peptidoglycan and muramic acid
(Bl Cellulose
(Cl Lipoproteins
None of the above
17. Which type of flagellation in bacteria IS non-pollar one?

(Al Monotrichous (Bl Lophotrichous
(Cl Peritrichous Amphitrichous
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IK. lTost common mode of multiplication III Bacteria is
(AI Endospore formation Binary fission
I Conidia formation Cyst formation
I IS a symbiotic association between
I Algae and Fungi (El Algae and Bacteria
CI Fungi and Root None of these
LU Which of the following culture media do not ha"e Agar-Agar
Solid media Semisolid media
Selective solid media Liquid broth
21. Mycovirus infects
Insects Fungi
Bacteria Cyanobacteria

22.
Which of the following IS a cyanobacteria

23.
Engulfment of a particulate matter by the cell is known as


(AI E.coli (BJ Salmonella
Ie! Anabaena (DJ Pseudomonas
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CAl Phagocytosis CB1 Penocytosis
CCl Mitosis CDl None of these
24. Who postulated the Germ theory of disease
CAl Tyndall CBl John Snow
CCl Robert Koch CD) Leeuwenhoek
25. Which of the following IS oxygenic cyanobacteria
CAl Spirulina CBl Shigella
CCl Rhizobium CDl Klebsiella
26. Find out the purple non-sulfur bacteria out of the following
CAl Thiocystis CBl Rhodospirillum
CCl Nitrobacter CDl Macromonas
27. The light Harvesting photosystems in purple bacteria ranges m between
the wavelength of
IAI 680-700 nm eEl 300-400 nm
800-870 nm CDl None of these
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28. Net yield of ATP during Glycolysis IS
2 (Bl 4
(Cl 8 10
29. EMP pathway is not found in
Arthrobacter Azotobacter
E.coli Thiobacillus
30. In which bacterium Entner-Doudoroff pathway of Glucose breakdown IS
found:
Xanthomonas Arthrobacter
Escherichia coli Azotobacter
31. Most methanogens grow well with
02 03
(Cl CO2 CO
32. Which of the following methanogens is Gram negative
Methanobacterium (Bl Methanocarcina
Methanobravibacter Methanococcus
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33. Which bacterium has a flexible cell wall composed of protein and traces of
Glucosamine
Methanococcus Azotobacter
E.coli CD) None of these
34. The bacteria which grow as at high temperature and low pH are known
Acidophiles Halophiles
Thermophiles Thermoacidophiles
35. 8ulfolobus rapidly oxidizes
36. H28 Cl2 Thermoplasma is a 802 None of these
tA) Facultative aerobe Facultative anaerobes
Obligate aerobe 8/80/2013/12 None of these P.T.O.

37.
What element is used as terminal electron acceptor by thermoproteus

(Al Cu (Bl Fe (Cl S Mg

38.
Desulfurococcus grow at the temperature in between (Al 75°-95°C (Bl 0°-16°C (Cl 10°-20°C (Dl None of these

39.
Which of the following is a monosaccharide (Al Glucose Sucrose (Cl Maltose None of these

40.
A common polysaccharide found in the plant cell wall is (Al Glycogen (Bl Cellulose (Cl Starch Glucose

41.
A heptose sugar contains (Al 6 carbon (Bl 7 carbon (Cl 5 carbon 4 carbon


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42. Out of the following which is not a sugar?
Ribose Ribulose
Xylulose Glyceraldehyde
43. Glycogen is a polysaccharide of
Cellulose Starch
Glucose None of these
44. Which of the following is a hctcropolysaccharide
Pectin Starch
Glycogen Cellulose
45. Starch gives blue colour with
Fehling's Solution (BJ Benedict Reagent
Tollens Reagent Iodine Solution
46. Which of the following is an G amino acid
Succinic Acid Fumaric Acid
(Cl Alanine Malic Acid
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47. A polypeptide is made up of

Units of organic acids


Units of fatty acids


48. Lock and Key hypothesis of enzyme action

Kuhne


Sumner


49. Which enzyme digest fats?

Lipase


Invertase


50. Apoenzyme is a

Fat


Protein


Units of amino acids None of these
was given by Emil Fischer Kirchhoff
Zymase None of these
Carbohydrate None of these
51. Natural Resistance against the infection is known as

Acquired immunity Susceptibility


Innate immunity None of these


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52. Which of the under-mentioned cells are involved in Immune system

Phagocytic cell Leucocytes


Lymphocytes All of them


53. Immunity can be acquired artificially by .

Vaccination


Antibodies


Placental Antibodies IgG and IgA


All of the above


54. Smallpox vaccine is prepared from

Vaccinia VIruS Bacillus bacteria


Mycoplasma Cyanobacteria


55. Inactivated toxins are used as vaccines and known as

Toxoids Live vaccmes


Killed vaccines None of these


56. Which of the following vaccines are administered intramuscularly?
Whooping cough vaccme DPT
Tetanus toxoid All of these 8/80/2013/12 P.T.O.
57. An agent which stimulates the production of antibodies or substance for immune response IS

Anticodon Antigen


Antidot CD) None of these


58. The specific surface on antigen where antibodies are fixed known as

Isotopes Heterotopes


Epitopes CD) None of these


59. Immunoglobulin-G has a structure of

Y-type L-type


H-type CD) Z-type


60. Which category of lymphocyte cells generate the immune response

B-cells T-cells


N.K. cells CD) All of these


61. In vertebrates the lymphocytes are produced from

Stem cells of bone marrow Cells of Lymph


Cells of Blood CD) None of these


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62.
Macrophages generated from haemopoetic stem cell is (Al Multikaryotic (El Dikaryotic (Cl Monokaryotic None of these

63.
Chemically the Immunoglobulins are (Al Glycoprotein (Bl Lipoprotein (Cl Thioprotein (Dl None of these

64.
How many classes of immunoglobulins have been recognized (Al 4 (El 3 (Cl 5 8

65.
The molecule of immunoglobulin is made up of


Only light polypeptide chain


Only heavy polypeptide chain
(Cl Both, light and heavy polypeptide chains



None of the above



66.
Which type of immunoglobulin IS having highest value III mg/lOO m!. of blood serum


IgG (Bl IgA
IgM IgD
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67. In vitro reactions of antibodies and antigens are termed as

Biological reactions Serological reactions


Inorganic reactions None of these


68. Which of the' following bacterium is Gram -ve and causal orgamsm of Diarrhea?

Bacillus anthracis Clostridium tetani


Salmonella typhimurium Mycobacterium leprae


69. Which of the following diseases is caused by Gram positive bacteria?

Diphtheria Leprosy


Tetanus All of these


70. Amebic dysentery is caused by a

Chlamydea Rickettsia


Protozoa Mycoplasma


71. Bacterial and viral diseases can be spreaded through

Water Air


Contact All of these


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72.
Typhoid fever is caused by CA) Salmonella CB) Escherichia CC) Amoeba CD) Blastomyces

73.
Mycosis is caused by CA) Aspergillus Candida CC) Mucor I DI of these

74.
Measles, mumps and Rubella is caused by a CA) Fungal agent CBl Viral agent CC) Bacterial agent CD) None of these

75.
Hepatitis is a I AI Heart Disease CB) Liver Disease

Renal Disease CD) Skin Disease

76.
DNA is found in CA) Nucleus Mitochondria CC) Chloroplast CD) All of these

77.
Which amino base is not found in RNA? CAl. Uracil CBl Guanine CC) Cytocene CD) Thymine


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72. Typhoid fever is caused by

Salmonella Escherichia


Amoeba Blastomyces


73. Mycosis is caused by

Aspergillus Candida


Mucor All of these


74. Measles, mumps and Rubella is caused by a

Fungal agent Viral agent


Bacterial agent None of these


75. Hepatitis is a

Heart Disease Liver Disease


Renal Disease Skin Disease


76. DNA is found in

Nucleus Mitochondria


Chloroplast All of these


77. Which amino base IS not found in RNA? Uracil Guanine
Cytocene Thymine
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p.T.a.

78. Double helix model of DKA was proposed by

Mesalson and Stahl Watson and Crick


M. Nirenberg Fischer and Heldan


79. In one Triplet codon how much amino bases are found?
2 6
3 4
80. DNA is replicated at the time of:
phase of Interphase phase of Interphase
phase of Interphase None of these 8l. The capacity of self-duplication is found Ill:

Ribose sugar Deoxyribose sugar


DNA None of these


82. Most evidenced method of DNA replication is

Dispersive method Conservative method


Semiconservative method Condensation method


83. Which mechanism of gene transfer is found in bacteria

Conjugation Transduction


Transformation All of these


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S.Jo. Who demonstrated the phenomenon of transformation in pneumococcus
CAl F. Griffith CBl Lederberg and Tautum CCl C.M. Macleod CD) None of these
85.
Transfer of F. plasmid during conjugation takes place between: CAl Donor and Donor cell CBl Recipient and Recepient cell CCl Donor and Recipient cell CD) None of these

86.
Transductional genetic transfer in bacteria involves alan ·CAl Bacteriophage CBl Cyanobacteria CCl Insect CD) None of these

87.
Which of the following phages does not cause lysogeny? CAl Tphage CB) PI phage


z CC) Lambda phage CD) None of these 68, Viroids are
CAl Double Stranded RNA CBl Single Stranded RNA Cl Single Stranded DNA CDl None of these
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89. During lysis of bacterial cell wall which compound IS hydrolysed (Al Peptidoglycan Cellulose
Chitin Pectin
90. Joining of DNA fragments IS known as

Condensation Suppression


Ligation Restriction


91. The process of mRNA synthesis by DNA tamplet is
Transformation (El Transduction
(Cl Translation (ol Transcription

92, Which of the following is used as scissor in recombinant gene technology? (Al Ligases (El Restriction endonucleases (Cl Insertases Gyrase
93, Plasmid with a fragment of COS DNA is known as
Phasmid Phragmid
Cosmid None of these
Gene responsible for the synthesis of nitrogenase enzyme IS

Ti Gene (Bl Nif Gene
(Cl Nut Gene None of these

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95. Gene cloning involves the steps

Isolation of gene
(El Insertion of gene in a vector



Transfer of recombinant gene in multiplying cell CD) All of the above


96. Recombinant gene technology is useful in the development of

Industrial enzymes Pharmacology


GMO development All of these


97. Plasmids are also known as

Episome Nucleosome


Chromosome CD) None of these


98. Out of the following which cosmid is used for gene cloning?

PJ37 (El PJC8


PJE8 CD) PJA7


99. In E.coli Nif gene is transferred from

Salmonella Agrobacterium


Klebsiella pneumonae None of these


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100. Some DNA sequences are capable of changing their positions and known
as
Preons Recons
Mutons Transposons
10l. Which of the following is not a zooplankton
Ceriodaphnia Navicula
Cyclops Brachionus
102. Which of the following is not a cyanobacteria
Oscillatoria Anabaena
Nostoc Hydrodictyon
103. In ocean, low temperature tolerating microbes are known
Heliophilic Barophilic
Psychrophilic None of these
104. Accumulation of microbial cells on polymeric matrix is know:: as
Biofilm Biochip
Biosurfactants None of these
105. The amount of oxygen required by microbes of sewage to stabilize organic
matter is known as
COD DO
BOD None of these
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106. Sewage treatment involves
Physical treatment Biological treatment
Chemical disinfection All of these
107. As per WHO Standards coliform count of any sample of 100 ml water is

100 75
200 o (zero)
108. Water can be disinfected by
NH3 S02
Cl2 None of these
109. Botulism is caused by
C. botulinum C. perfrigens
E.coli S. aureus
110. Which organism produce aflatoxin
Salmonella Clostridium
Aspergillus Shigella
111. Food poisoning by microbes causes
Paralysis Vomiting
Diarrhoea All of these
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112. Cholera in human beings is caused by
(AJ Streptococcus pyogenes E.coli
Shigella Vibrio
113. Poisonous compound produced by fungi are known as

Phycotoxin Shigatoxin


Mycotoxin Cytotoxin


114. What are the common contaminants of the air?
Spores Conidia
Chlamydospores All of these
115. Which of the following IS an air-borne disease
Tuberculosis Hepatitis
Polio Enteric fever
116. White rust of crucifer is an air-borne disease of
Plant Animal
Human beings None of these
117. Which of the following microbes IS a phosphate biofertilizer

Pseudomonas Rhizobium
Nitrosomonas CD) Azotobacter
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118. Which of the following is a symbiont of Azolla
Scytonema Lyngbya
Anabaena Oscillatoria
119. Out of the following which organism is used in making of cheese?
Streptococcus lactis Penicillium candidum
S. cremoris All of these
120. Chloramphenicol antibiotic is produced by
Streptomyces griseus S. venezuelae
S. erythreus None of these
121. Penicillin is produced by a
Bacteria Virus
Fungi Algae
122. Which of the following is not a dairy product
Yoghurt Curd
Cream Toast
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123. Ethanol and CO2 IS produced from sugars by 128. Steam. sterilization of media is done in
(Al Fusarium (Bl Salmonella
(Cl Alternaria Saccharomyces
124. Antibiotic acts on
(Al Wall of pathogen (Bl Cell membrane of pathogen
(Cl Inhibition of protein synthesis All of these
125. Brewing industry is based on
(Al Pasteurization (Bl Jerovization
(Cl Fermentation and distillation (Dl None of these
126. Most popular method of isolation of pure culture IS
(Al Pour plate method (Bl Spread plate method
(Cl Streak plate method (Dl None of these
127. Methods used for preservation of pure culture
(Al Drying III vacuum (Bl Lyophilization
(Cl Saline suspenSIOn (Dl All of these
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Laminar air flow Hot air oven
Incubator Autoclave
129. In saccharomyces which enzymes are responsible for fermentation
(Al Invertase, Zymase (Bl Lipase, Protease
Diastase, Maltase Catalase, Pectinase
130. Rectified spirit is
(Al 50% Ethanol 30% Ethanol
(Cl 95.5% Ethanol None of these
131. Coffey's Still equipment is a
Fermenter Centrifuge
Distillation unit Electronic Balance
L32. For the continuous culture which type of bioreactor is used
(Al Open type Closed type
(Cl Closed stirred tank None of these
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133. Large bioreactors are used at
Domestic scale Industrial scale
Academic laboratory scale None of these
134. Screening of microorganism which are able to produce growth factors IS
known as
Crystallography Auxenography
Radiography None of these
135. The innoculum cells can be separated from the medium by
Centrifugation
Sedimentation
Purification by single cell culture
All of the above
136. By which test microbial limit of milk is judged
Fat test Widal test
MBR test None of these
137. Yoghurt is also termed as
Sour milk Ordinary curd
Bulgarian milk . Swiss milk
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138. The milk is rendered safe by high temperature exposure for short time, the
process is known as
(Al Purification (Bl Putrification
(Cl Pasteurization Fermentation
139. Which of the following is a good food preservative
(Al Potassium metabisulphate Magnesium carbonate
(Cl Formadehyde None of these
140. Annular plate and diaphragm IS found in
(Al Dissecting microscope (Bl Sterioscopic microscope
(Cl Phase-contrast microscope None of these
141. Electromagnetic lenses are used III
Fluorescence microscope (Bl Electron microscope
(Cl Compound microscope None of these
142. Spectrocolorimeter is based on

(Al Beer-Lambert's Law Newton's Law
Dalton's Law None of these
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143. Which of the following is single cell protein
(AJ Spirulina (BJ Yeast
(CJ Scendesmus All of these
144. Sauerkraut is a fermented food prepared from:
(AJ Pineapple (BJ Cabbage
(CJ Olives Cucumbers
145. In thin layer chromatography
(AJ Glass plate and silica gel is used
Wattman paper is used
(CJ Solvent column is used
None of the above
146. Chromatography involves
(AJ Loading of sample on stationary phase
(BJ Separation by moving phase
(CJ Elution of the separated component
All of the above
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147. In which of the chromatography mobile phase is liquid and stationary phase
is solid
TLC
Paper chromatography
Gel filtration chromatography
I,Dr All of the above
148. Wide range of wavelength can be used in
Nephalometer Spectrophotometer
IC) Lavibond comparator Colorimeter with four filters
149. Bacterial cell mass is measured in terms of
I Ar Absorbancy
I B r Intensity of light falling on suspenSlOn
I Optical darkness
IDr None of the above
150. Yitamin BI2 is produced from
(AI Pseudomonas denitrificans
(BJ Bacillus coagulens
Propionibacterium freudenreichii
All of the above
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