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KlT 2 A

A 3 KlT
1. The molar concentration of a solution of chemical species is the number of moles of that species that is present in

one litre of the solvent one cm3 of the solution


one cm3 of the solvent one litre of the solution


2. Concentration in ppb is the
mass of solute mass of solution x 106 mass of solute mass of solution x 109
mass of solute mass of solution x 103 mass of solute mass of solvent x 109

3. The molarity of a K3Fe(CN)6 solution that contains 63·3 ppm of K3Fe(CN)6 (329·3 gmImol) is

0·1922 M 5·2022 M


1·922 x 10-4M 1·922M


4.
2 M Sulphuric acid solution is equivalent to

5.
Crystalline precipitate should ordinarily be digested for longer duration on steambath in order to avoid the problem of


Co-precipitation


Post precipitation


Co-precipitation and post precipitation both


Dissolution of the precipitate





6. is the closeness of measurement to other measurement made III
exactly the same way.
Precision Accuracy Deviation Error

7. The boundaries of confidence interval are called

Significance limit Confidence limits


Confidence range Significance level


8. The chemical analysis is affected by at least types of errors.
indeterminate determinate
None of these All of these

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p.T.a.
KLT 4 A
9. Mohr's method is used for estimation of Nitrate ions Fluorine ions Chloride ions Sulphate ions

10. Non-aqueous ionizing solvents can sometimes be used in acidimetry and alkalimetry. In which one can the solvent encourage the loss of proton from a weak acid making it effectively much stronger?

Polar solvent Non-polar solvent


Acidic solvent Basic solvent


11. The following indicator solution is called as adsorption indicator

Tartrazine Fluorescein


Methyl red None of the above


12. Primary standard solution should contain a substance which is

highly pure stahle in air


more soluble in water All of the above


13. If the precipitate is a salt of weak acid and strong base and is slightly soluble, it may exhibit a tendency to hydrolyse and the soluble product of hydrolysis will be a

base acid


neutral polar


14. Washing of precipitate in gravimetric analysis is more advantageous if precipitate is washed with given amount of solvent with

more amount of solvent each time but less number of washing


less amount of solvent each time but more number of washing


one washing with all the solvent


None of the above


15. The contamination of the precipitate by substances, which are normally soluble in the mother liquor is referred to as

Digestion Coagulation


Precipitation Co·precipitation


16. Occlusion means

slow precipitation


crystal formation


precipitate settles down


a compound is trapped within a pocket during rapid crystal growth


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A 5 KLT
17. PPMA may be expressed as (mass of A/mass of solution) x 106 (mass of A/mass of solution) x 10-6 (mass of A/mass of solvent) x 106 (mass of Ajmass of solvent) x 10-6

18. The solubility product of AgCI and AgI are 1·2 x 10-10 and 1·7 x 10-16. When silver nitrate solution is added in solution containing nitrate, chloride and iodide ion, which salt will be precipitated first?

Silver chloride Silver iodide


Silver nitrate All of the above


19. An assay is a process of determination of

error


of sample


how much of given sample is the material indicated by its name


None of the above


20. The best indicator for titration of HCI with NH40H is

Diphenylamine Methyl orange


Phenol red Phenolphthalein


21. Metallochrome indicators are
strong acids strong bases
weak acids weak bases


22. How many grams of KMn04 will be required to prepare 1000 cm3 of 1 N solution?
(M. wt. of KMn04= 158)
31-6 g 158·0 g 69·0 g 15·8 g
23. The indicator Eriochrome Black T is red in the pH range

4·7 to 6·7 7·7 to 10·8


11·2 to 12·4 12·7 to 13·2


24. In replicate analysis of a metal ore the following results were obtained
Sample No. 1 2 3 4 5 6
of metal 55·13 55·16 55·14 55·15 55·17 55·15

Hence the mean is
55·17 55·13 55·14 55·15
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p.T.a.
KLT 6 A
25. When large excess of SnClz is added to HgClz soLution, the substance formed is HgzClz Sn Hg None of the above

26. The region between is known as quartz region.
150 nm to 300 nm 150 nm to 380 nm
200 nm to 380 nm 200 nm to 300 nm



27. In AAS nitrous oxide acetylene flame is preferred to produce ground state atoms for those elements which are

d-block elements form more refractory compounds


for mercury for f-block elements



28. In AAS the ratio of the number of atoms to the total number of analyte species, atoms, ions and molecules in the flame is always

one one or less than one


infinity depend on mist


29. In the spectrophotometry titration of a copper ion solution with EDTA may be
carried out at a wavelength of nm.
420 470 560 745

30. The sensitivity of AAS is higher than flame emission spectroscopy because

flame emission spectroscopy obeys Beer-Lambert's law at only very low concentration


AAS depends on the absorption of radiation by atoms in ground state


in FES number of atoms in excited state is higher than ground state


reasons recorded in and


31. UV-visible spectra of condensed system are useful as

vacuum region far region


fingerprint region large region


32. In spectrophotometers the intensity of transmitted light is determined by

photoelectric cell tungsten lamp


monochromator ampJ'ifier


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A 7 KLT
33. In electrogravimetric methods platinum electrodes are generally used because they are relatively reactive they are relatively non-reactive their current density increases their current density decreases

34. The emission of radiation of a single wavelength corresponding to a definite energy transition is known as

spectral lines band lines


line reversal discrete spectral lines


35. Which is made up of a series of closely spaced lines and is due to molecular species in the vapour state?

Line spectra Band spectra


Emission spectra Absorption spectra


36. An indifferent electrolyte used in direct current polarography means

which neither conducts current nor reacts with material under investigation and eiectrode


which conducts current but does not react with electrode and material


which does not conduct current but reacts with electrode


which does not conduct current but reacts with material under investigation


37. According to Ilkovic, the diffusion current Id depends upon

temperature


viscosity of the medium and molecular or ionic state of active species


the dimension of the capillary and pressure of dropping mercury


All of the above


38. The diffusion current is proportional to

decomposition potential


residual current


concentration of electrolytic solution


concentration of electro-active material present in the solution


39. In Amphometric titration of an iodide solution with mercury nitrate solution, the magnitude of the anodic diffusion current

decreases upto the end point


till all the active material is reduced


increases as the titration proceeds


reaches to its maximum


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P.T.O.


KlT 8 A
40. In stripping voltammetry during electrolysis certain process gives rise to species which form an insoluble salt with Hg ions. In the subsequent process, the voltage scan must be in the negative direction and so that procedure is referred to as

anodic stripping voltammetry


cathodic stripping voltammetry


dropping mercury voltammetry


None of the above


41. In stripping voltammetry the rate of amalgam formation is governed by the

magnitude of the current flowing


concentration of reducible ions


rate at which the ions travel to the electrode


All of the above



42. An electron beam passing through an evaculated region can be focused by means of

magnetic field electric field


magnetic field or electric field None of the above


43. The instruments required for the following techniques are closely related to microscopy

Electron diffraction X-ray projection microscopy


X-ray microprobe analyser All of the above



44. In electrogravimetry good metallic deposits are

Spongy Fine grained


Powdery Flaky


45. The strongest absorption for dienes, polyenes, aldehydes and ketones result from electronic transitions.
cr cr* cr n n
46. Cyclohexanone exhibits only peaks in its 13C NMR spectrum due to
symmetry.
Two Four Three Five


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A 9 KLT
47. In flame emission, the intensity of emission is critically dependent upon

the rate at which the sample is introduced


the nature of the sample


the state of the sample


None of the above


48. In cyclic voltammetry, a cyclic voltammogram for a chemically reversible system shows

equal peak heights for reduction and oxidation processes


unequal peak heights for reduction and oxidation processes


no peak heights


broader peaks


49. Amperometric titrations cannot be carried out at potentials more negative than 2 volts since
Nitrogen is evolved Hydrogen is evolved
Oxygen is evolved None is evolved
50. The GLC is limited to

Non-polar materials Polar materials


Volatile materials Non-volatile materials


51. In Raman spectra the middle line is called as

Raman lines Functional group lines


Rayleigh lines Peak lines


52. In IR spectra fingerprint region is from 1 11

1500 cm-1 to 1000 1300 cm-to 667 cm­11


1400 cm-1 to 1100 5000 cm-1 to 1300 cm­


53. If an organic compound does not absorb UV-visible radiation, it means that
compound does not contain
Single bond Sigma bond
Conjugated double bond Dative bond

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P.T.O.

KLT 10 A
54. The liquid mixtures, having distillation with a change in composition are called as non-equilibrium mixtures azeotropic mixtures zeotropic mixtures fractional distillation

55. Paper treated with silicone or paraffin oil permits paper chromatography.

reversed phase chemical phase


physical phase normal phase


56. The Raman shift depends upon
frequency of the incident light frequency of the scattered light
frequency of the Raman lines None of the above

57. The characteristics of lines observed in Raman effect is

the intensity of Stokes line is always greater than the corresponding anti-Stokes lines


Raman shift lies within the far and near regions of the spectrum


they are symmetrically displaced about the parent lines


All of the above
58. Intensity of Raman peak depends upon

Polarizability of the molecule Intensity of the source


Concentration of the active group All of the above


59. The efficiency of fractionating column of distillation is expressed in tenns of HETP and usually stated in
mm cm2 em m2
60. In chromatography, the ratio of distance travelled by the substance and standard substance from the origin is expressed in tenns of


61. Generally a solvent or solvent mixture selected as paper developing solvent which can give RF value in the range of

0·2 -0·8 0·0 -0·5


0·01 -0·05 0{12 -0·2


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A 11 KlT
62. In two-component chromatogram of chromatography, separation can be improved by

increasing the rate of band separation


decreasing the rate of band spreading


overlapping of peaks All of the above
63. The time between sample injection chromatogram is sometimes called Retention time Dead time and the appearance of detection of peak Migration time Distribution constant In

64. In chromatographic separation, a process in which species migrate from a more concentrated part of a medium to a more dilute region is known as

Migration Dispersion


Diffusion Extraction



65. In chromatography when the stationary phase is immobilized liquid, the mass-transfer coefficient is

directly proportional to square of the film on support particles


directly proportional to the concentration of solute injected


inversely proportional to square of thickness of the solute in the film


None of the above



66. In gas chromatography the detector widely used to detect halogenated compound is

Flame ionization detector Electron capture detector


Thermionic detector Photoionization detector


67. In Raman spectroscopy the wave number of Stokes line is
.6.v v.-V v
1S BI




68. The process of locating analytes on a thin layer plate is often termed as

Mobilization Visualization


Normalization None of the above


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P.T.O.
KLT 12 A
69. Exhaustive extractions can be performed with a extractor. simple fractional soxhlet counter current

70. In chromatography analyte species are adsorbed into the surface of a polar packing.
absorption adsorption ion-exchange paper
71. Thin layer chromatography is based on
absorption adsorption
solubility fractional distillation

72. In a mass spectrometer an organic compound is bombarded with electrons of about energy.
65 eV 70 eV 75eV 60 eV
73. Scanning Electron Microscopy provides morphologic and topographic information about

the surface of liquid the surface of solid


the surface of gas None of the above


74. Efficiency of a column in fractional distillation can be increased by increasing

number of bulbs in the column length of the column


width of the column All of the above


75. In paper chromatography stationary phase and mobile phase are

liquid -liquid liquid -solid


liquid -gas solid -gas


76. One millimolar solution mM) is equivalent to
1 l-l mol/ml 1 l-l mol/l 1 l-l mol/dl 1 m mol/dl
77. Widely used buffer for separation of proteins and other biological substances is
Phosphate buffer Tris-Borate buffer with EDTA
Cacodylate buffer Piperazine buffer
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A 13 KLT
78. Separation of proteins by isoelectric following factors Charge PI focussing technique depends on which of the Size Solubility

79. Universal blood donors have in their blood

no antigenic determinants on RBC and both Anti A and Anti B in plasma


both antigenic determinants on RBC and no antibodies in plasma


no antigenic determinants on RBC and no antibodies in plasma


both A and B antigenic determinants on RBC and both Anti A and Anti B in plasma


80. A ligand (hormone) for a receptor of interest is chemically linked to polystyrene beads. After affinity elution which of the following are you expected to collect?

Molecules of hormone Molecules of purified receptors


Hormone-receptor complex Assorted membrane receptors


81. Our body has many systems for cellular defence. Which of the following enzymes protect us against reactive oxygen species?

Xanthine oxidase Mono oxygenase


Dioxygenase Superoxide dismutase


82. The DNA-polymerase used in PCR for amplification of DNA fragments is specifically obtained from

Escherichia coli Thermus aquaticus


Hemophilus influimzae Rd Mammalian DNA-pol-alpha


83. How much stock solution. of glucose (0·1 will you require to prepare 10 ml solution of 1 mM glucose concentration [Mol. wt. of glucose is 180]
1 ml 0·1 ml 0·01 ml 0·001 ml
84. If Hill co-efficient of an enzyme is found to be the enzyme manifests

positive substrate cooperativity


negative substrate cooperativity


both types of substrate cooperativity


no cooperativity


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P.T.O.
KlT 14 A
85. Libermann-Burchard test is performed to detect Cholesterol· Triglycerides Fatty acids Glyc"rol
86. A positive Seliwanoff's test is obtained with Glucose Fructose Galactose Mannose

87. Polymerase Chain Reaction is useful for
cutting DNA into fragments creating recombinant plasmids
analysing person's fingerprinting making many copies of DNA

88. The electrophoresis technique that separates amphoteric compounds like proteins in a medium possessing a stable pH gradient is callE!d

SDS-PAGE Isotachophoresis


Iso-electric focussing High voltage electrophoresis


89. Age and rancidity of oil can be determined by method of

Iodine number Benedict test


Acid value Salkowski test



90. The fractional range of matrix for gel filtration is 1000 to 100,000. Those proteins that will not be separated by this bed will

penetrate beads completely elute in void vol


not penetrate beads at all All of the above


91. The pore size of polyacrylamide gel can be controlled by the concentration of

acrylamide bis-acrylamide


TEMED acrylamide and bisacrylamide


92. Diphenylamine method is employed in the quantitkation of
DNA RNA Proteins Carbohydrates
93. An allosteric modulator influences enzyme activity by
competing for the catalytic site with the substrate
binding to a site distinct from catalytic site
changing the nature of products formed
covalently modifYing enzyme

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A 15 KLT
94. Restriction Fragment Length Polymorphism (RFLP) technique is useful for which of the following

Isolation of genes with known location and function


Providing DNA for PCR analysis


Prenatal diagnosis of certain genetic defects


Increasing the small quantity of DNA available for further analysis



95. Restriction enzymes widely used in genetic engineering, belong to one of the
following classes
Oxidoreductase Transferase
Hydrolase Lyase

96. Which of the following is not true for cDNA

It lacks exons


Used for reproducing eukaryotic genome in prokaryotes


Produced by reverse transcriptase


Lacks regulatory elements of gene



97. Method that uses Coomassie Brilliant Blue dye for protein estimation is

Lowry method Kjeldahl method


Bradford's method Biuret method


98. The DNA polymerase that has both, the polymerisation and the 'primase' activity is

Prokaryotic DNA pol-I Prokaryotic DNA pol-III


Eukaryotic DNA pol-alpha Eukaryotic DNA pol-delta


99. The antigenic individuals is determinant saccharide on the erythrocytes of blood group
N-acetyl Galactose amine N-acetyl Glucose amine
D-Galactose D-Mannose

100. For radioimmuno assay radioisotopes that are generally used are

1311 1251


3H All of the above


SPACE FOR ROUGH WORK

P.T.D.
KLT 16 A
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