Seat No.: Enrolment
GUJARAT TECHNOLOGICAL UNIVERSITY
B.PHARM SEMESTER EXAMINATION -WINTER 2018
Subject Code: 2280015 Date: 28/11/2018
Subject Name: Genetic engineering and gene therapy
Time: 02:30 PM TO 05:30 PM Total Marks: 80
Instructions:
1. Attempt any five questions.
2. Make Suitable assumptions wherever necessary.
3. Figures to the right indicate full marks.
Q.1

Explain Gene Cloning with neat and labelled diagram.
06

Write a note on applications of genetic engineering.
05

What is central dogma of life? Explain the process of translation with diagram.
05
Q.2

What are vectors in genetic engineering? Enumerate the types of vectors. Explain plasmid PBR322 as a vector in detail with diagram.
06

What is RNA? Write a note on types of RNAs.
05

What is PCR explain the cycle and write applications of PCR.
05
Q.3

What do you mean by Antisense oligo nucleotide therapy?
06

What are monoclonal antibodies? How they are produced? Write the applications of Monoclonal Anti bodies.
05

What is blotting? Explain southern blotting with suitable diagram.
05
Q.4

What are transgenic plants? How they are produces? Write the applications of transgenic plants.
06

What is Humulin? Explain the production using recombinant DNA technology.
05

What do you mean by gene therapy? Explain the types with suitable example.
05
Q.5

Explain DNA delivery methods in detail.
06

Write a note on Quantification of Nucleic acids.
05

Explain in detail about transgenic animals.
05
Q. 6

Write in detail about DNA sequencing techniques. Explain any one technique using suitable diagram.
06

Write a note on transformation with diagram.
05

Explain about DNA vaccine in detail.
05
Q.7

Explain the application of gene therapy in Hematopoietic disorders.
06

What are restriction enzymes? Explain the types and applications in detail.
05

Explain about bacteriophage with suitable diagram.
05