Seat No.: Enrolment
GUJARAT TECHNOLOGICAL UNIVERSITY
B.PHARM SEMESTER EXAMINATION -WINTER 2018
Subject Code:2250003 Date: 27/11/2018
Subject Name: Pharmaceutical Analysis III
Time:10:30 AM TO 01:30 PM Total Marks: 80
Instructions:
1. Attempt any five questions.
2. Make Suitable assumptions wherever necessary.
3. Figures to the right indicate full marks.
Q.1

Classify Spectroscopy. Define Line Spectra, Isosbestic point and Stray Light.
06

State and Explain Lambert-Beer's Law with factors causing deviation from law.
05

Write a Short Note on: Gratings
Simultaneous Equation Method
05
Q.2

Explain different types of Stretching Bending Vibrations in IR Spectroscopy.
06

Differentiate Dispersive IR and FT IR.
05

Write a Short Note on: Finger Print Region
Sample Preparation Techniques.
05
Q.3

Enlist Ionisation Techniques in Mass Specroscopy. Explain any two in detail.
06

Give a brief account of Mc Lafferty Rearrangement TOF Mass Analyser.
05

Explain different types of peaks observed in Mass spectrum.
05
Q.4

Describe principle instrumentation of NMR Spectroscopy.
06

Define Chemical Shift. Give factors affecting Chemical shift.
05

Explain Spin splitting, Spin-Spin coupling Coupling Constant.
05
Q.5

Explain working principle of Fluorimeter with well labeled diagram.
06

Differentiate Fluorescence Phosphorescence. Draw explain Jablonski Diagram.
05

Explain in brief factors affecting Fluorescence Intensity.
05
Q. 6

Describe Qualitative and Quantitative applications of UV-Visible Spectroscopy.
06

Enlist Radiation Sources Detectors used in IR Spectrosopy. Explain any one detector in detail.
05

Differentiate AAS and AES.
05
Q.7

Explain working principle of Flame Photometer with labeled diagram.
06

Comment on following:
Fluorescence Spectroscopy is more sensitive than UV-Visible
Spectroscopy.
Base peak in mass spectrum is peak of highest mass.
(iii)C13 NMR spectra are more difficult to record than H-NMR.
Fluorescence occur at shorter wavelength than Absorbance.
J value does not depend on applied magnetic field.
05
PTO

Give λmax value of following compounds.
CH2
C H3 CH3
05