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Seat No.: Enrolment
GUJARAT TECHNOLOGICAL UNIVERSITY
ME SEMESTER IV EXAMINATION SUMMER 2018
Subject Code: 2743004 Date:- 02/05/2018
Subject Name: Bioprocess &Biochemical Engineering
Time: 02:30 PM To 05:00 PM Total Marks: 70
Instructions:
1. Attempt all questions.
2. Make suitable assumptions wherever necessary.
3. Figures to the right indicate full marks.
Q.1

Distinguish between prokaryotic and eukaryotic cells. Explain the salient features of different types of eukaryotes and proakryotes with more elaboration.
07

Starting from the basic equation between enzyme and substrate derive the Michaelis-Menten equation for the enzyme catalyzed reaction. State the assumptions clearly. How do you determine the kinetic parameters of the above equation?
07
Q.2

Illustrate primary, secondary and tertiary structure of proteins with schematic. What is protein denaturation. State the biological functions of proteins.
07

Classify carbohydrates with examples and salient features. What are the functions of carbohydrate in cellular organism?
OR
07

Derive the design equations for continuous stirred tank fermenter Draw the schematic and state the assumptions. What do you understand by critical dilution rate and wash out conditions?
Q.3

Aerobic degradation of an organic compound by a mixed culture of organisms in waste water can be represented by the following reaction:
C3H6O3 aO2 bNH3 cC5H7NO2 d H2O e CO2
Determine d and if Yx/s 0.4 g X/g S.
ii) Determine the yield coefficient s Yx/O2 and Yx/NH3
07

Discuss the power requirement in sparged and agitated vessels for biochemical reactions.
07
OR
Q.3

A liquid medium in a batch fermenter having working volume 1000L is sterilized in situ at 122oC. The initial concentration of bacterial spores in the medium was found to be 5 105 The sterility level after the holding cycle of sterilization is desired not to exceed 10 Initial temperature of the medium is 32oC and during heating cycle 10% of microorganism is expected to be destroyed. Calculate the holding time. Additional data given (Thermal death rate constant α 8.0 1038 E 29 J/kmol
07

State and explain the principle of centrifugation for cell separation in fermentation. Explain with a diagram and example how various
07
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organelles of eukaryotic cells can be separated by differential centrifugation.
Q.4

Discuss various methods of cell disruption for separation of intracellular products. Compare their merits and demerits.
07

Discuss thermal death rate kinetics during sterilization of microbial cells. What is the difference between sterilization and Pasteurization?
07
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Q.4

Discuss various components of media preparation with examples. Give examples of different solid media.
07

Discuss the advantages and disadvantages of continuous culture of microorganism.
07
Q.5

What are the advantages of enzyme immobilization? Discuss with diagrams different methods of enzyme immobilization.
07

With the help of a schematic diagram enlist various gas-liquid mass transfer resistances in cellular system.
07
OR
Q.5

State various methods of determination of volumetric mass transfer coefficient and explain any one in details.
07

Discuss modulation and regulation of enzyme activity. Explain competitive, non competitive and uncompetitive inhibition.