FACULTY OF PHARMACY
M. Pharmacy (Common to All) I-Semester (Suppl.) Examination,
August 2018
Subject: Modern Pharmaceutical Analytical Techniques
Time: 3 Hours Max. Marks: 75
Note: Answer any five questions. All questions carry equal marks.
1 Discuss the instrumentation of double beam UV visible spectrophotometer
with a neat labeled diagram.
What is Isobestic point? Explain with a labeled UV spectrum giving tow
examples.
2 Compare the instrumentation and working a dispersive and foruier transform
IR spectrometers. Write the advantages and disadvantages of the two
techniques.
Draw a schematic IR spectrum for any one compound and indicate the
absorption wave number regions for any four functional groups in the
compound.
3 Explain
Chemical shift and factors influencing chemical shift.
Spin-spin coupling and coupling constant.
Draw a schematic HNMR spectrum for any one compound and explain the
following:
Chemical shift values Nature of protons Number of protons
4 Discuss the theory and principle of mass spectroscopy and explain the
instrumentation and working of mass spectrometer with a neat labeled
diagram.
What is fragmentation? Explain the following by taking a simple example
Fragmentation peaks Molecular ion peak Base peak
5 Discuss the theory of HPLC. Describe the instrumentation and working
of HPLC with a neat labelled diagram.
Draw a schematic HPLC chromatomgram and explain
Retention time Resolution Peak Asymmetry
6 Discuss the theory and principle of electrophoresis. Explain the method of
capillary electrophoresis and its applications with examples.
What is isoelectric focusing?
7 Discuss the theory and principle of Gas chromatography. Explain the
instrumentation and working of Gas chromatography and explain various
stationary and mobile phases used in GC.
How non voralile compounds can be analysed by GC. Explain the technique
with few examples?
8 Write a note on
Flame emission spectroscopy
Instrumentation and application of Florescence spectroscopy