Total No. of Questions 12] [Total No. of Pages 2
M.Sc. (Previous) DEGREE EXAMINATION, MAY 2018
First Year
MICROBIOLOGY
Microbiological Methods
Time 3 Hours Maximum Marks 70
SECTION A × 6 30)
Answer any Five of the following
Q1) Physical methods of sterilization
Q2) Chemical methods of sterilization
Q3) Brewer Jar
Q4) Pyrogallic acid
Q5) TLC
Q6) Applications of HPLC
Q7) Beer's lambert Law
Q8) GM counter
SECTION B × 10 40)
Answer all questions of the following
Q9) Describe the principle, Methodology and applications of TEM.
OR
Describe the composition and preparation of bacteriological media.
Q10) Write an account on general methods of isolation of Bacteria.
OR
Describe the methods of preservation and maintenance of microbial cultures.
Q11) Describe isolation and purification of Viruses.
OR
Write an account on the principles of Centrifugation.
Q12) Describe two dimentional and pulse field gel electrophoresis.
OR
Describe the principle, methodology and applications of IR spectroscopy.