M.Sc. (Second) DEGREE EXAMINATION, MAY 2018
BOTANY
Second Year
Plant Biotechnology
Time 3 Hours Maximum Marks 70
SECTION A × 6 30)
Answer any Five questions from the following
Q1) Tissue culture techniques
Q2) Selection of mutants in vitro for biotic stress.
Q3) Synthetic seeds
Q4) Cell suspension
Q5) c DNA libraries
Q6) PCR
Q7) Transgenic plants
Q8) Gene transfer methods
SECTION B × 10 40)
Answer all questions
Q9) How do you produce haploids through anther culture?
OR
Write an essay on meristem culture and embryogenesis.
Q10) By using tissue culture as a tool how do you produce secondary metabolites?
OR
Describe protoplast fusion and somatic hybridization.
Q11) Enumerate the salient features of in vitro genetic engineering.
OR
Describe the different blotting techniques studied by you.
Q12) Explain the role of RFLP and RAPD in crop improvement.
OR
Describe the Agrobacterium mediated gene transfer.