(Pages 1621
P.T.O.
MAR IVANIOS COLLEGE (AUTONOMOUS)
THIRUVANANTHAPURAM
Reg. No. :.………………… Name :.…………………
First Semester Career Related B.Sc. Degree Examination, November 2016
First Degree Programme under CBCSS Complementary Course: Biochemistry I (for Botany and Biotechnology)
AUBB131: Introduction to Biochemistry
Common for Regular 2016 Admn. and Improvement 2015 Admn.
Time: 3 Hours Max. Marks: 80
SECTION A
Answer ALL questions in one or two sentences.
1. State the Bronsted theory of acids and bases.
2. Define normality.
3. Define isotonic and hypertonic solutions.
4. What are emulsions Give examples of emulsifying agents.
5. Define entropy and enthalpy.
6. State Beer Lambert's law.
7. Write the principle of centrifugation technique.
8. Define partition coefficient.
9. How is NaOH prepared
10. What is mean by a glycosidic bond
(10 1 10 Marks)
SECTION B
Answer any EIGHT questions, not exceeding a paragraph.
11. Calculate the pH of a 0.001M solution of HCl.
12. Distinguish between molality and molarity.
13. What is diffusion and write its biological significance.
14. State Vant Hoff's law of osmotic pressure.
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15. What are colloids
16. Explain the term Gibbs free energy.
17. What is molar extinction coefficient
18. Comment on the structure of water.
19. What are the advantages of TLC over paper chromatography
20. Write note on Rf value.
21. What are the different functional groups in biomolecules
22. Write note on Watson Crick base pairing.
2 16 Marks)
SECTION C
Short essay type Answer any SIX questions.
23. Briefly discuss the buffers in biological systems.
24. Draw and discuss the titration curve of a weak acid.
25. Write the biological importance of osmosis and surface tension.
26. Explain Donnan membrane equilibrium.
27. Write note on energy changes in biochemical reactions.
28. Write note on the components and working of spectrophotometer.
29. Explain isopycnic centrifugation.
30. Explain the principle and applications of gel filtration chromatography.
31. Discuss the working principle of pH meter.
4 24 Marks)
SECTION D
Long essay type Answer any TWO questions.
32. Derive Henderson Hasselbalch equation for a weak acid.
33. What are colloids Describe the classification and properties of colloids.
34. Discuss the principle, procedure and applications of differential centrifugation.
35. Discuss the principle, procedure and applications of SDS PAGE.
15 30 Marks)