(Pages: 1485
P.T.O.
MAR IVANIOS COLLEGE (AUTONOMOUS)
THIRUVANANTHAPURAM
Reg. No. :.………………… Name :.…………………
Fifth Semester Career Related B.Sc. Degree Examination, November 2016
First Degree Programme under CBCSS Vocational Course VIII: (for Botany and Biotechnology)
AUBB552: Plant Biotechnology
Time: 3 Hours Max. Marks 80
SECTION A
Answer ALL the following in a word or one or two sentences.
1. Cybrid
2. Two chemical surface sterilants
3. Electroporation
4. Auxins
5. Edible vaccines
6. Somaclones
7. Haploids
8. Cell immobilisation
9. Synthetic seeds
10. Totipotency
(10 1 10 Marks)
SECTION B
Answer any EIGHT questions, not exceeding one paragraph.
11. Write a note on cell suspension culture
12. Differentiate between differentiation and dedifferentiation.
13. What is meristem culture
14. Define somaclonal variation. Mention its applications.
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15. What are solidifying agents Give examples.
16. Comment on hairy root culture.
17. What are microprojectiles
18. How are protoplasts isolated
19. What is activated charcoal Where is it used
20. Macro nutrients and micro nutrients.
21. What is T-DNA
22. Maintaining suitable PH is important in tissue culture. Why
2 16 Marks)
SECTION C
Short essay type Answer any SIX questions.
23. What is MS medium Write a note on preparation of MS medium
24. Give an outline of metabolic engineering of plants for various products.
25. Explain Agrobacterium mediated gene transfer in plants.
26. What are GM crops What are the environmental concerns regarding GM crops
27. Define callus culture. Mention its applications.
28. Explain briefly somatic embryogenesis.
29. Describe different types of suspension cultures.
30. Write a note on the surface sterilisation of explants.
31. Comment on growth regulators.
4 24 Marks)
SECTION D
Long essay type Answer any TWO questions.
32. Enumerate various stages in micropropagation and discuss its advantages and disadvantages.
33. Discuss the various methods of the production of Haploid plants. Add note on its applications.
34. What is somatic hybridization What is its significance Explain the steps involved in somatic hybridization.
35. Briefly explain various methods of direct gene transfer in plants.
15 30 Marks)