BIOTECH/V/CC/11 Student's Copy
2 0 1 8
CBCS
5th Semester
BIOTECHNOLOGY
SIXTH PAPER
Recombinant DNA Technology
Full Marks 75
Time 3 hours
PART A—OBJECTIVE
Marks 25
The figures in the margin indicate full marks for the questions
SECTION—A
Marks 10
Tick the correct answer in the brackets provided 1×10=10
1. The process of binding of primers to the denatured DNA strands is called
denaturation
annealing
renaturation
None of the above
2. What is the function of polynucleotide kinase?
Addition of g-phosphate at 3¢ -OH
Addition of g-phosphate at 5¢ -OH
Removal of g-phosphate at 3¢ -OH
Removal of g-phosphate at 5¢ -OH
/155 1 Contd.
3. l ZAP vector is an example of
phage
phagemid
cosmid
plasmid
4. Plasmid incompatibility is inability of
a plasmid to grow in the host
two different plasmids to coexist in the same host cell
Both and
None of the above
5. The method widely used for transforming animal cell cultures using lipid
vesicles or liposomes is
lipotransformation
liposome-mediated transformation
lipofection
lipid-mediated DNA transfer
6. Transformation carried out using a particle gun is known as biolistic
transformation. It falls under which category of transformation?
Physical
Chemical
Electroporation
Natural
7. Which of the following is not a function of reverse transcriptase?
RNA-dependent DNA polymerase
DNA-dependent DNA polymerase
RNase H
Exonuclease
BIOTECH/V/CC/11/155 2 Contd.
8. Choose the correct statement for genomic libraries
Genomic libraries include the representation of the whole genome of the
organism
Sequences such as telomeres are also represented
Telomeres can be readily cloned
The larger the size of insert of genomic DNA in recombinants, the more
is the number of recombinants required to represent the genome in the
library
9. Which of the following is untrue about DNA sequencing methods?
Purified fragments of DNA cut from plasmid/phage clones or amplified
by polymerase chain reaction
Clones of DNA fragments are denatured to single strands, and one of
the strands is hybridized to an oligonucleotide primer
Taq polymerase is quite heat-sensitive
New strands of DNA are synthesized from the end of the
primer
10. Guanine specific cleavage in Maxam Gilbert method is done by using
formic acid
hydrazine
dimethyl sulphate
piperidine
SECTION—B
Marks 15
Write short notes on/Answer the following 3×5=15
1. Technique of DNA labelling
OR
Composition and conditions of a polymerase chain reaction procedure
BIOTECH/V/CC/11/155 3 Contd.
2. General characteristics of cloning vectors
OR
Components of M13-based vector
3. Importance of Type-II restriction endonuclease
OR
Blunt and cloning
4. What are the advantages of chromosome walking?
OR
Differentiate between Southern blotting and Northern blotting.
5. Shot-gun method of gene sequencing
OR
Automated DNA sequencing method
PART B—DESCRIPTIVE
Marks 50
The figures in the margin indicate full marks for the questions
1. Write the principles and applications of recombinant DNA technology.
5+5=10
OR
Describe different types of polymerase chain reaction methods and their
uses. 7+3=10
2. Elaborate on the construction and applications of expression vectors.
5+5=10
OR
Differentiate between phagemids and cosmids. 10
3. Describe microinjection and gene-gun method of gene delivery. 10
BIOTECH/V/CC/11/155 4 Contd.
Or
Write short notes on the following 5+5=10
Ligases
Phosphatases
4. Describe the procedure and application of Western blotting. 5+5=10
OR
What is cDNA library? Explain the mechanism of preperation of a cDNA
library. 2+8=10
5. Explain the Sanger method of DNA sequencing. 10
OR
What is DNA fingerprinting? Describe the process and applications of DNA
fingerprinting. 2+8=10