BIOTECH/III/EC/05 Student's Copy
2 0 1 8
CBCS
3rd Semester
BIOTECHNOLOGY
Paper BT-III
Microbiology
Full Marks 75
Time 3 hours
PART A—OBJECTIVE
Marks 25
The figures in the margin indicate full marks for the questions
SECTION—A
Marks 10
Tick the correct answer in the brackets provided 1×10=10
1. Alexander Fleming is known for the discovery of
transforming principle
RNA structure
penicillin
DNA structure
2. Who is known as the 'Father of Microbiology'?
Robert Hooke
Edward Jenner
Louis Pasteur
Joseph Lister
/56 1 Contd.
3. Which of the following is a symbiont with human?
Helicobacter pylori
Amoeba
Propionibacterium
Escherichia coli
4. Binomial nomenclature system is invented by
R. H. Whittaker
Carolus Linnaeus
Louis Pasteur
Edward Jenner
5. Before autoclaving a liquid media in a conical flask, the ideal volume of the
media should be
1
2
volume of conical flask
1
3
volume of conical flask
1
4
volume of conical flask
None of the above
6. Agar is used as a
nitrogen source carbon source
solidifying agent nutrient source
7. Which of the following is a xenobiotic compound?
Biofertilizer Chlorophyll a
Pesticide None of the above
8. In gram staining, which of the following acts as a mordant?
Crystal violet Iodine
Safranin Ethanol
BIOTECH/III/EC/05/56 2 Contd.
9. The generation time of Escherichia coli is
20 minutes 30 minutes
40 minutes 50 minutes
10. The most appropriate stage for harvesting microbial cells for physiological
experiment is
lag phase log phase
stationary phase death phase
SECTION—B
Marks 15
Write short notes on the following 3×5=15
1. Pasteurization
OR
Tyndallization
2. Structure of a bacterial cell
OR
Asexual reproduction
3. Selective media for bacterial growth
OR
Differences between solid and liquid media
4. Serial dilution technique
OR
Functions of endospore
5. Importance of incubation temperature for growth of microbes
OR
Effect of UV light on microbial growth
BIOTECH/III/EC/05/56 3 Contd.
PART B—DESCRIPTIVE
Marks 50
The figures in the margin indicate full marks for the questions
Answer all questions
1. How the idea of spontaneous generation got started? Describe the
experiment conducted by Spallanzani and salient findings. 4+6=10
OR
What do you understand by germ theory of disease? State Robert Koch's
postulates, and mention how his postulates were used for treating
tuberculosis. 2+8=10
2. What are extremophiles? Describe the cellular structure of archaea
bacteria. 2+8=10
OR
Discuss the classification of algae. 10
3. What do you understand by sterilization? Describe the various physical
sterilization techniques. 2+8=10
OR
Outline the various steps for media preparation. Write a brief note on the
applications of MacConkey agar media. 4+6=10
4. What is enrichment technique? Describe giving suitable examples how it
can be used for isolation of microorganisms. 2+8=10
OR
Write short notes on the following 5+5=10
Capsule staining
Flagella staining
5. Describe the different stages of microbial growth curve. Derive an equation
for calculating generation time. 10
OR
Write a note on the various methods by which bacterial growth can be
monitored. 10