BIO/VI/09 Student's Copy
2 0 1 8
6th Semester
BIOTECHNOLOGY
Paper BT-IX
Plant Biotechnology
Full Marks 55
Time 2½ hours
PART A—OBJECTIVE
Marks 20
The figures in the margin indicate full marks for the questions
SECTION—A
Marks 5
Tick the correct answer in the brackets provided 1×5=5
1. Phyllode is present in
Australian acacia
Opuntia
Asparagus
Euphorbia
/525 1 Contd.
2. A naturally occurring auxin (growth regulator) is
6-benzylaminopurine
indoleacetic acid
indolebutyric acid
zeatin
3. Disease-free plants are produced by
seed culture
meristem tip culture
root tip culture
embryo culture
4. Cryopreservation is done at which of the following temperatures?
-196 °C
-126 °C
-96 °C
-100 °C
5. The identification and selection of several genomic regions for complex trait
within a single population is done by
marker assisted selection
marker assisted backcrossing
marker assisted recurrent selection
marker assisted cross selection
BIO/VI/09/525 2 Contd.
SECTION—B
Marks 15
Write notes on the following 3×5=15
1. Morphology of stems
2. Inorganic nutrients for tissue culture
3. Various stages of embryogenesis
4. Gene sanctuaries
5. Strategies of avoiding gene silencing
PART B—DESCRIPTIVE
Marks 35
The figures in the margin indicate full marks for the questions
1. Give an illustrated note on structure of a flower. Describe the
reproductive processes in flowering plants.
OR
Write a note on plant defence mechanism. 7
2. Write a note on the role of plant growth regulators in tissue culture
experiments. 7
OR
Describe the major steps involved in plant tissue culture technology.
BIO/VI/09/525 3 Contd.
3. Write a note on organogenesis by callus formation and direct
adventitious organ formation. 3½+3½=7
OR
Write notes on the following 3½+3½=7
Axillary bud culture
Meristem culture
4. Write a note on various strategies used for crop improvement. 7
OR
What are cryoprotectants? Name the steps involved in cryopreservation.
Write a note on the use of cryoprotectants.
5. What is gene silencing? Write a note on the application of gene
silencing.
OR
Describe in brief the nuclear genome organization in plants. 7