BIOTECH/II/EC/03 Student's Copy
2 0 1 8
CBCS
2nd Semester
BIOTECHNOLOGY
SECOND PAPER
Biochemistry
Full Marks 75
Time 3 hours
PART A—OBJECTIVE
Marks 25
The figures in the margin indicate full marks for the questions
SECTION—A
Marks 10
Tick the correct answer in the brackets provided 1×10=10
1. The glycolytic enzyme belongs to which of the following groups of enzyme?
Oxidoreductases
Lyases
Hydrolases
Isomerases
2. Induced fit hypothesis of enzyme is proposed by
Emil Fisher
Ralph F. Hirschmann
Daniel Koshland
None of them
/318 1 Contd.
3. One molecule of glucose metabolized through glycolysis, Krebs cycle and
electron-transport chain will yield
30 ATP
32 ATP
34 ATP
36 ATP
4. In eukaryotes, reaction of citric acid cycle takes place in
cytosol
membrane
nucleus
mitochondria
5. Inosine monophosphate is the immediate precursor for the
formation of
UMP
AMP
CMP
ATP
6. The major site for purine nucleotide synthesis is
liver
heart
brain
spleen
7. Which of the following is not a nitrogen fixer?
Bacillus
Clostridium
Klebsiella
Azotobacter
8. 3-phosphoglycerate serves as a precursor for the biosynthesis of
arginine
tryptophan
methionine
glycine
BIOTECH/II/EC/03/318 2 Contd.
9. Adipose cells act as a site for storage of
carbohydrates
triglycerides
proteins
None of the above
10. Which of the following molecules serves as a backbone for phospholipids
biosynthesis?
Glycine
Guanine
Glycerol
Ethanol
SECTION—B
Marks 15
Write short notes on the following 3×5=15
2. Enzyme classification
OR
Activation energy
3. Characteristics of metabolic pathway
OR
ATP synthase
4. Thymine synthesis
OR
GS-GOGAT cycle
5. Ammonification
OR
Cumulative feedback control
6. Ketosis
OR
Functions of Triacylglycerols
BIOTECH/II/EC/03/318 3 Contd.
PART B—DESCRIPTIVE
Marks 50
The figures in the margin indicate full marks for the questions
1. Describe the molecular mechanism of enzyme action. 10
OR
Derive Michaelis-Menten equation. What is the significance of Km values?
8+2=10
2. Write a note on gluconeogenesis. 10
OR
Give a detailed account of Krebs cycle. Write a chemical equation summing
up the whole cycle. 1+9=10
3. What do you understand by salvage pathway? Write a note on the salvage
pathway for purine and pyrimidine nucleotides. 1+9=10
OR
Explain in brief the catabolism of purine nucleotides. 10
4. Discuss the biosynthesis of amino acids from ketoglutarate precursor
family. 10
OR
What is the role of urea cycle? Briefly explain the steps involved in it.
1+9=10
5. Write short notes on the following 5×2=10
Carnitine shuttle
Prostaglandin
OR
Write a note on the biosynthesis and regulation of fatty acids. 10