BC/IV/04 Student's Copy
2 0 1 8
Pre-CBCS
4th Semester
BIOCHEMISTRY
Paper BC-4
Molecular Biology
Full Marks 55
Time 2½ hours
PART A—OBJECTIVE
Marks 20
The figures in the margin indicate full marks for the questions
SECTION—A
Marks 5
Tick the correct answer in the brackets provided 1×5=5
1. During the course of DNA replication, the proof-reading function is carried
out by the enzyme
DNA polymerase III
DNA ligase
primase
RNA polymerase
2. Intervening nucleotide sequences in mRNA which do not code for proteins
are
inteins
exons
introns
exteins
/415 1 Contd.
3. Those genes whose products (proteins) are required all the time in a cell
are known as
inducibile genes
housekeeping genes
repressible genes
inhibitor genes
4. Post-translational modifications include all the following, except
glycosylation
hydroxylation
decarboxylation
phosphorylation
5. The first pharmaceutical product of rDNA technology approved for human
use is
insulin
GH
interferon
hepatitis B vaccine
SECTION—B
Marks 15
Write short notes on any five of the following 3×5=15
1. Replication fork
2. Okazaki fragments
3. Promoters
4. Capping
5. Features of genetic code
6. Shine-Dalgarno sequence
7. Gene cloning
BC/IV/04/415 2 Contd.
PART B—DESCRIPTIVE
Marks 35
The figures in the margin indicate full marks for the questions
1. Describe in detail the experimental evidence for DNA as genetic
material. 7
OR
Write short notes on the following 3½×2=7
RNA primer
DNA polymerase III
2. Explain in detail the post-transcriptional processing of RNA in
eukaryotes. 7
OR
Write short notes on the following 3½×2=7
RNA polymerase
Reverse transcriptase
3. Discuss in brief the Lac operon model of E. coli. 7
OR
Write short notes on the following 3½×2=7
Genes within genes
Biological significance of degeneracy of genetic code
BC/IV/04/415 3 Contd.
4. Explain in detail the translation in prokaryotes. 7
OR
Write short notes on the following 3½×2=7
Structure of ribosomes
Activation of amino acids
5. Describe the recombinant DNA technology. What are the important
applications of the technique?
OR
Write short notes on the following 3½×2=7
Plasmids
Monoclonal antibodies