FINAL EXAM NATIONAL BOARD OF EXAMINATIONS
DECEMBER 2017
POSSESSION/USE OF CELL PHONES OR ANY SUCH ELECTRONIC GADGETS IS NOT PERMITTED INSIDE THE
EXAMINATION HALL.
BIOCHEMISTRY
PAPER IV
Time 3 hours BIO/D/17/03/IV
Max. Marks 100
Important instructions:
• Attempt all questions in order.
• Each question carries 10 marks.
• Read the question carefully and answer to the point neatly and legibly.
• Do not leave any blank pages between two answers.
• Indicate the question number correctly for the answer in the margin space.
• Answer all the parts of a single question together.
• Start the answer to a question on a fresh page or leave adequate space between two
answers.
• Draw table/diagrams/flowcharts wherever appropriate.
Write short notes on:
1. Types of stem cells and their therapeutic potential. 10
2. What is gene therapy? How does it work? Explain gene
transfer vehicles.

3. High Pressure /Performance Liquid Chromatography (HPLC)
and its advantages.
6+4
4. Molecular cloning and its applications.
6+4
5. Compare and contrast methods /applications of Radio
Immunoassay and enzyme linked immunoassay.
10
6. DNA fingerprinting
RT-PCR (Reverse Transcriptase Polymerase Chain
Reaction)
5+5
7. Flow cytometry
Mass spectrometry
5+5
8. SnRNAs
Micro RNAs
5+5
9. HIV vaccine
Anti-obesity vaccine
5+5
10. Micro array technique
Antisense therapy
5+5