1
LOYOLA COLLEGE (AUTONOMOUS), CHENNAI 600 034
M.Sc. DEGREE EXAMINATION MEDICAL LAB TECHNOLOGY
THIRD SEMESTER NOVEMBER 2017
16PML3MC01 MOLECULAR BIOLOGY AND HUMAN GENETICS
Date: 01/11/2017 Dept. No. Max. 100 Marks
Time: 09:00-12:00
Section A
Answer ALL the questions (10×2= 20 Marks)
1. What is the central dogma of molecular biology?
2. List down the different forms of DNA.
3. Expand VNTR and SNP.
4. Define pyrosequencing.
5. What is the advantage of pedigree analysis?
6. Give a brief note on karyotyping.
7. Write short notes on linkage.
8. Define genetic marker.
9. What is meant by haemoglobinopathy?
10. Write a brief note on epigenetics.
Section B
Answer any FOUR of the following (4×10 40 Marks)
11. Write short notes on DNA replication.
12. Explain PCR and its applications.
13. Expand RAPD RFLP and add notes on its applications.
14. Write an account on chromosome and its functions.
15. Explain linkage analysis.
16. How a single gene disorder affects human? Explain it with an example.
Section C
Answer any TWO of the following (2×20 40 Marks)
17. Write in detail about the translation and post translational modification of proteins.
18. Give a detailed account on DNA sequencing methods.
19. Explain molecular genetic markers.
20. Types of chromosomal disorders- Discuss.