1
LOYOLA COLLEGE (AUTONOMOUS), CHENNAI 600 034
M.Sc. DEGREE EXAMINATION BIOTECHNOLOGY
THIRD SEMESTER NOVEMBER 2017
BT 3825 BIOPROCESS PHARMACEUTICAL BIOTECHNOLOGY
Date: 08-11-2017 Dept. No. Max. 100 Marks
Time: 09:00-12:00
PART A
Answer ALL the Questions
I. Choose the correct answer x 1 5 Marks)
1. Yield coefficient represents
biomass or product produced conversion efficiency of a substrate into product
conversion rate of a substrate into product production time of product
2. Which of the following is not physical method for the cells rupturing?
milling homogenisation ultrasonication enzymatic digestion
3. Impellers used in fermentors help in
aeration agitation antifoaming absorption
4. Muromonab CD 3 is used for
reversal of kidney transplant rejections anaemia
prevention of blood clotting diabetes mellitus
5. Alteplase is used to
dissolve blood clots increase insulin production
control blood pressure treat cystic fibrosis
II. State whether the following are true or false, if false, give reason (5x1=5 Marks).
6. The productivity of a continuous fermentation is less than that of batch fermentation.
7.Red algae used as food is porphyra.
8. Peptide mapping is done for identification of proteins.
9. Sulfur dioxide is added to wine during its production to enhance the growth of lactobacilli.
10. Nutritional deficiency is the factor which causes the accumulation of organic acids in
fermentation
III. Complete the following x 5 Marks)
11.Primary metabolites are produced during
12. Citric acid is recovered by adding
13. L glutamic acid is produced by direct fermentation.
14.Hydro carbon of interest in the bacterialSCP production is
15. Phenolic acids are potent
IV. Answer the following, each within 50 words x 1 5 Marks)
16. What are the rules to be followed while doing scale up studies?
17. How ionizing radiations cause mutation?
18. Define auxostat.
19.How are fermented milk products classified?
20. What is indigenous fermentation?
2
PART B
Answer the following, each within 500 words. x 8 40 marks)
Draw diagram wherever necessary
21. What is a fermenter? Give the basic structure of a fermenter.
OR
Write briefly on the different methods of cell disruption in downstream processing.
22. Discuss strain improvement based on mutation and Recombinant DNA Technology
OR
Give the structural details and application of fluidized bed reactor and tower
Fermenter.
23. Write short notes on liquid liquid extraction, liquid solid extraction
OR
Write about the different types of centrifuges used in bioprocessing industries.
24. Write in detail on targeted drug delivery? Add a note on different type of delivery
vehicles.
OR
Write a short note on the production of recombinant insulin.
25.Give a brief account of the clinical development of the first therapeutic antibody.
OR
Discuss the role of FDA in clinical trials.
PART C
Answer any TWO of the following, each within 1500 words. x 20 40 Marks)
Draw diagrams wherever necessary.
26.Write in detail on the methods and maintenance of industrially important cultures.
27. What are the different types of cheese? Explain in detail the production of cheese.
28. Write in detail on target drug delivery.
29. Explain bioanalytical method validation and its pharmaceutical application.