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LOYOLA COLLEGE (AUTONOMOUS), CHENNAI 600 034 M.Sc.DEGREE EXAMINATION BIOTECHNOLOGY FIRSTSEMESTER APRIL 2017
16PBT1MC03- MICROBIOLOGY
Date: 04-05-2017 Dept. No. Max. 100 Marks
09:00-12:00 PART A Answer ALL the Questions
I. Choose the correct answer x 1 5 Marks)
1. Evolutionary relationships between groups of organisms are determined using which of the following type of information?
Comparisons of nucleotide sequences Comparisons of biochemical pathways
Comparisons of structural features All of the above
2. Desulfovibrio desulfuricansis a typical example of autotroph organotroph chemotroph mixotroph
3. Gram-negative bacteria, responsible for food poisoning Salmonella Pseudomonas c)Clostridia Corynebacteria
4. The process of erythrocytes adhering to the infected cells is called as Haemagglutination Haem adhering Haemadsorption Haem absorption
5. Sexual spores are formed by mitotis division meiotic division binary dividion fusion.
II. State whether the following are true or false (5x1=5 Marks)
6. The three domain version of life on earth is based on the nucleic acid sequence data.
7. Mesosomes are present in both gram positive and negative bacteria.
8. Pneumococci are sensitive to optochin.
9. Cell line produced using oncogenic viruses are called as heteroploid.
10. Chitin is a major polysaccharide in fungal cell wall.
III. Complete the following x 5 Marks)
11. Haeckel proposed the kingdom unicellular organisms.
12. Carboxysomes are involved in
13. Yaws are produced by
14. The protein free, virus like agents are called
15.The endospores seen inside sporangium are called as
IV. Answer the following, each within 50 words x 1 5 Marks) 16.What is bacteriorhodopsin? Where is it found?
17. What are the modifications seen in a Phase Contrast Microscope.
18. What is Quelleng reaction?
19. List the functions of the viral structural proteins.
20. What is mycetoma?
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PART B Answer the following, each within 500 words. Draw diagrams wherever necessary. (5×8 40 Marks)
21. (a)Write briefly on the experiments conducted to disprove spontaneous generation. OR Describe Whittaker's five kingdom concept. Adda note on its merits and demerits.
22. Discuss the structure of a bacterial cell wall. What are the major differences between the cell wall composition of gram positive and gram negative cell wall. OR Write a short note on the different phases of bacterial growth curve.
23. Write briefly on the virulence factors and pathogenesis of Neisseria meningitides. OR Discuss the clinical manifestations of Gonorrhoea.
24. (a)Briefly describe the basic steps in the viral life cycle. OR Discuss the structure, transmission and symptoms of TMV.
25. Write a short note on candidiasis OR What is cutaneous mucoses? Discuss with an example.
PART C Answer any TWO of the following, each within 1500 words. Draw diagrams wherever necessary. (2×20 40 Marks)
26. Explain the structure, function of SEM and TEM. Add a note on sample preparation.
27. Enlist the virulence factors of Staphylococcus aureus. Describe the various conditions caused by this bacterium along with their laboratory diagnosis.
28. Write in detail on the lytic and lysogenic cycle of bacteriophages.
29. Illustrate the life cycle of malarial Parasites.