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LOYOLA COLLEGE (AUTONOMOUS), CHENNAI 600 034
M.Sc.DEGREE EXAMINATION BIOTECHNOLOGY
SECONDSEMESTER APRIL 2018
17PBT2ES02- BIOANALYTICAL TECHNIQUES
Date: 25-04-2018 Dept. No. Max. 100 Marks
Time: 01:00-04:00
PART A
Answer ALL the Questions
I. Choose the correct answer x 1 5 Marks)
1. High speed refrigerated centrifuge operate at maximum
150,000 g 100,000g 200,000 g 10,000 g
2. The separation technique of charged molecules under influence of an electric current
Dot blot Electrophoresis Western Blot ELISA
3. Infra-red radiation has a wavelength
1mm-700nm 1nm-700nm 700nm-1500nm 1cm-700mm
4. Technique which uses electric nebulization of liquid that results in formation of charged droplets
Electrospray MALDI
Scintillation counterr AAS
5. Technique used to detect abnormal heart rythm
ERG ECG CT ESI
II. State whether the following are True or False. (5x1=5 Marks)
6. Greater the size of particle to be sedimented, greater the gravitational force.
7. Capillary electrophoresis is a tool for studying properties of single charged species.
8. Luciferase is a gene for chemiluminescence.
9. X-ray diffraction determines the atomic structure of crystals. 10. Electroretinogram is a test in which the electrical potentials generated by the retina of the eye are measured.
III. Complete the following x 5 Marks)
11. Common feature in all centrifuge
12. Process of passing the mobile phase through the column is
13. is a difference in the length of a bond.
14. Geiger-Müller tube is filled with an inert gas such
15. sensors are used in ECG.
IV. Answer the following within 50 words x 1 5 Marks)
16. What are the different zones in a sedimentation tank?
17. Mention any two uses of native PAGE.
18. What is Planck's constant?
19. Which kinds of radiation are detected by Geiger Miller tube?
20. What is an invivo imaging study?
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PART B
Answer the following each within 500 words. x 8 40 Marks)
Draw diagrams wherever necessary.
21. Describe the principle and working of a radial flow sedimentation tank.
OR
Write a note on different methods of cell disintegration.
22. Give an account on paper chromatography. OR
Explain the principle and theory of capillary electrophoresis.
23. Describe the wave properties and particle properties of electromagnetic waves. OR
Write a brief account on fluorescence spectroscopy.
24. Discuss the working of liquid scintillation counter.
OR
Write briefly about the electrospray ionization technique.
25) Give notes on computed tomography.
OR
What is an electroretinogram? Briefly explain the principle of an electroretinogram.
PART C
Answer any TWO of the following, each within 1500 words. x 20 40 Marks)
Draw diagrams wherever necessary.
26. Give an account on differential centrifugation technique.
27. Write an essay on pulse field gel electrophoresis.
28. Discuss the instrumentation, principle and working of atomic absorption spectroscopy.
29. Describe the working method, theory and applications of MALDI-TOF.