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LOYOLA COLLEGE (AUTONOMOUS), CHENNAI 600 034
M.Sc.DEGREE EXAMINATION BIOTECHNOLOGY
FIRSTSEMESTER APRIL 2018
17PBT1MC04- IMMUNOLOGY
Date: 30-04-2018 Dept. No. Max. 100 Marks
Time: 09:00-12:00
PART A
Answer ALL the Questions
I. Choose the correct answer x 1 5 Marks)
1. are the most abundant leukocytes in a normal WBC count.
Neutrophils Eosinophils Basophil Macrophage
2. Immunoglobulin genes are located on
2 14, 22 22 14, 21
3. is a systemic autoimmune disorder.
Rheumatoid arthritis Myasthenia Gravis Diabetes Hydrocephalus
4. Which of the following vaccine is effective against certain types of cervical cancer? Vaccinia Gardasil Hep B Rotarix
5. Immunofluorescence is a technique that employs
Fluorophore fluorescence microscopy labeled antibodies all of the above
II. State whether the following are True or False. (5x1=5 Marks)
6. Kupffer cells are phagocytic cells present in the liver.
7. Tonegawa was awarded the Nobel Prize for his discovery of the genetic principle for generation of antibody diversity.
8. Serum sickness is an example of Type 1 hypersensitivity.
9. Vaccines are typically administered intravenously.
10. Immunosensors are solid-state biosensors where the immunochemical reaction is coupled to a transducer.
III. Complete the following x 5 Marks) 11. Active MAC has a subunit composition of
12. The diversity region is present only the in chain of an immunoglobulin.
13. typing is a technique to match a patient and donor for transplantation compatibility. 14. The first vaccine was developed
15. is a technique that determines antibody levels using antigens tagged with a radioisotope.
IV. Answer the following within 50 words x 1 5 Marks)
16. Define hapten.
17. What are oncogenes?
18. Name the type of transplant between genetically non-identical twins.
19. Give an example of a live attenuated vaccine.
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20. Name a immunodiagnostic technique that is based on the principle of Immunoprecipitation.
PART B
Answer the following each within 500 words. x 8 40 marks)
Draw diagrams wherever necessary
21. Describe the structure of an immunoglobin.
OR
Write a note on the types and functions of antibodies.
22. Describe the structure of MHC class I molecule and add a note on its function. OR
Explain HLA typing and its importance in transplantation.
23. Briefly explain the mechanisms by which tumors evade immune responses.
OR
Give an account on the types of grafts.
24. Write a note DNA vaccines used in cancer treatment/prevention.
OR
Explain hybridoma technology for the production of monoclonal antibodies.
25. Describe the technique of western blotting.
OR
Explain Ouchterlony Double diffusion.
PART C
Answer any TWO of the following, each within 1500 words. x 20 40 Marks)
Draw diagrams wherever necessary.
26. Describe the cells of the immune system and their functions.
27. Explain the exogenous pathway of antigen processing and presentation.
28. Outline the types of Hypersensitivity reactions with examples.
29. Write an essay on the types of ELISA and its applications.