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LOYOLA COLLEGE (AUTONOMOUS), CHENNAI 600 034
M.Sc.DEGREE EXAMINATION BIOTECHNOLOGY
FIRSTSEMESTER APRIL 2018
17PBT1MC02- BIOCHEMISTRY
Date: 02-05-2018 Dept. No. Max. 100 Marks
Time: 01:00-04:00
PART A
Answer ALL the Questions
I. Choose the correct answer x 1 5 Marks)
1. What is the pH of 0.1M HCl?
0 1 7 14
2. Which of the following chromatographic techniques is used to separate based on charge?
Ion exchange Affinity chromatography
Gel filtration Size exclusion chromatography
3. The enzyme that converts phosophoenol pyruvate to pyruvate is
Aldolase b)Enolase c)Pyruvate kinase Hexokinase
4. Which among the following is a membrane bound enzyme?
a)Citric acid synthase Aconitase
c)Succinate dehydrogenase d)Fumarase
5. Pick the substrate that acts as a competitive inhibitor for methanol poisoning?
Butanol Isopropanol Malic acid Ethanol
II. State whether the following are True or False. (5x1=5 Marks)
6. Ethanol is not soluble in water.
7. The peptide bond has a partial double bond character.
8. Oxygen is the final electron acceptor in electron transport chain.
9. Mevelonate is an intermediate in cholesterol synthesis pathway.
10. Arabidopsis thaliana has a very short lifecycle.
III. Complete the following x 5 Marks)
11. The molarity of pure water is
12. the simplest amino acid.
13. Glycolysis yields ATP.
14. The proteins to be degraded are attached to
15. are enzymes that transfer of specific groups from one substrate to the other.
IV. Answer the following within 50 words x 1 5 Marks)
16. What is proton hopping?
17. Give an example for an unsaturated fatty acid?
18. Define oxidation.
19. Mention the two pathways for nucleotide synthesis.
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20. Give an example for cofactor.
PART B
Answer the following each within 500 words. x 8 40 marks)
Draw diagrams wherever necessary
21. (a)Mention any four properties of water.
OR
Derive the Henderson Hasselbalch equation.
22. Classify carbohydrates with one example each. OR
(b)Discuss the structural hierarchy of proteins.
23. Outline the steps involved in Glycolysis.
OR
Comment on any two factors affecting hydrolysis of ATP.
24. Explain protein degradation pathway.
OR
Write notes on transamination and deamination reactions.
25. Mention any four classes of enzymes and their function.
OR
Explain competitive inhibition with a suitable example.
PART C
Answer any TWO of the following, each within 1500 words. x 20 40 Marks)
Draw diagrams wherever necessary.
26. Describe the principle and working of pH meter.
27. Elaborate on Ion exchange chromatography and affinity chromatography.
28. Write in detail about electron transport chain.
29. Explain synthesis and degradation of fatty acid.