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LOYOLA COLLEGE (AUTONOMOUS), CHENNAI 600 034
B.Sc.DEGREE EXAMINATION -PLANT BIOLOGY AND PLANT BIOTECHNOLOGY
FIFTH SEMESTER APRIL 2018
PB 5518 PB 5504 PLANT BIOTECHNOLOGY
Date: 17-04-2018 Dept. No. Max. 100 Marks
Time: 09:00-12:00
PART A
Answer the following, each within 50 words: (10 x 2 =20marks)
1. Define explant.
2. Define callus.
3. What issomoclonal variations?
4. Define cybrid.
5. What are nifgenes?
6. Define Ti-plasmid.
7. What are restriction enzymes?
8. Define molecular probe?
9. Expand RFLP and mention its importance.
10. What are transgenics?
PART B
Answer the following, each within 500 words. Draw diagrams and flowcharts wherever necessary: X 7 35marks)
11.a. List out the importance of plant tissue culture

b. Write the composition of MS medium. Add a note on the role of hormones in tissue
culture
12.a. Explain the steps involved in cryopreservation.

b. How are artificial seeds produced under in vitroconditions.
13.a. Explain the structure and organization of mitochondrial DNA

b. Give a brief note on genetic organization of Ti-plasmids
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14.a Explain the steps involved in PCR and mention its applications.

b. Write short notes on genomic library
15.a Explain briefly about RAPD and its applications.

b. Write the importance of molecular markers in genomic mapping.
PART C
Answer any three of the following, each within 1200 words. Draw diagrams and flowcharts wherever necessary: X 15 45marks)
16. Write an essay on types of sterilization used in plant tissue culture.
17. Illustrate the steps involved in isolation and fusion of protoplasts.
18. Explain the mechanisms involved in transformation of plants by Agrobacterium
tumefaciens 19. Explain the different types of blotting techniques?
20. Describe the development of transgenic plants with reference to herbicide resistance.