M.Sc. DEGREE EXAMINATION, NOVEMBER 2017
Fourth Semester
Botany
METHODS IN BIOLOGY
(CBCS 2011 onwards)
Time 3 Hours Maximum 75 Marks
Part A (10 x 2 20)
Answer all questions.
Write short notes on
1. DNA chip.
2. AFLP.
3. Autoradiography.
4. Antiserum.
5. Spectrum.
6. Circular dichroism.
7. Protein databases.
8. Sequence retrieval.
9. Resolving power.
10. Negative staining.
Sub. Code
1MBO4C2
AFC-10553
2
Ws5
Part B x 5 25)
Answer all the questions, choosing either or
11. Briefly describe the procedure for preparing a cDNA
library.
Or
Explain the principle, procedure and applications of
RAPD.
12. What is immunofluorescence and for what purpose
this technique is used?
Or
Explain the principle, procedure and applications of
genomic in situ hybridization.
13. Mention some of the medical applications of ESR
spectroscopy.
Or
Write the principle and applications of UV-visible
spectroscopy.
14. Give an account on genome analysis tools.
Or
Describe any four data bases available in public
domain.
15. Write notes on light microscopy.
Or
Enumerate different methods of fixation of
biological samples for electron microscopy.
AFC-10553
3
Ws5
Part C x 10 30)
Answer any three questions.
16. Describe the procedure and applications of micro arrays
technique.
17. Describe liquid scintillation counter.
18. Describe the different types of mass spectrometry.
19. Elaborate the applications of multiple sequence
alignment tools.
20. Illustrate the principle, procedure and applications of
SEM.
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