Exam Details
| Subject | recent trends in microbiology | |
| Paper | ||
| Exam / Course | ph d | |
| Department | ||
| Organization | solapur university | |
| Position | ||
| Exam Date | 28, June, 2017 | |
| City, State | maharashtra, solapur |
Question Paper
Ph.D. Course Work Examination June July 2017
MICROBIOLOGY (Paper II)
Recent Trends in Microbiology
Day Date: Wednesday, 28-06-2017 Max. Marks: 100
Time: 11.00 AM to 02.00 PM
Instructions: All questions are compulsory.
Figures to the right indicate full marks.
Draw neat and well labeled diagrams
wherever necessary.
Q.1 Explain in detail principle, working and applications of Atomic
Absorption Spectroscopy.
10
Write short notes on any two: 10
NMR
X-ray Crystallography.
IR
Q.2 Elaborate on various steps involved in GCMS. 10
Write short notes on any two: 10
Thin Layer Chromatography.
Applications of radioisotopes in biology.
UV/ Visible Spectroscopy.
Q.3 Describe schematically various steps in Density gradient
centrifugation.
10
Write short notes on any two: 10
Ion exchange chromatography
SDS- PAGE
High Performance Liquid Chromatography
Q.4 Discuss on principle, types applications of Polymerase Chain
Reaction.
10
Write short notes on any two: 10
Southern blotting.
Centrifugation preparative analytical.
DNA fingerprinting.
Q.5 Write in detail 16-s r-RNA gene sequencing. 10
Write short notes on any two: 10
Lipid profiling FAME analysis.
Restriction mapping -RFLP.
Phylogenetic backbone for prokaryotic systems.
MICROBIOLOGY (Paper II)
Recent Trends in Microbiology
Day Date: Wednesday, 28-06-2017 Max. Marks: 100
Time: 11.00 AM to 02.00 PM
Instructions: All questions are compulsory.
Figures to the right indicate full marks.
Draw neat and well labeled diagrams
wherever necessary.
Q.1 Explain in detail principle, working and applications of Atomic
Absorption Spectroscopy.
10
Write short notes on any two: 10
NMR
X-ray Crystallography.
IR
Q.2 Elaborate on various steps involved in GCMS. 10
Write short notes on any two: 10
Thin Layer Chromatography.
Applications of radioisotopes in biology.
UV/ Visible Spectroscopy.
Q.3 Describe schematically various steps in Density gradient
centrifugation.
10
Write short notes on any two: 10
Ion exchange chromatography
SDS- PAGE
High Performance Liquid Chromatography
Q.4 Discuss on principle, types applications of Polymerase Chain
Reaction.
10
Write short notes on any two: 10
Southern blotting.
Centrifugation preparative analytical.
DNA fingerprinting.
Q.5 Write in detail 16-s r-RNA gene sequencing. 10
Write short notes on any two: 10
Lipid profiling FAME analysis.
Restriction mapping -RFLP.
Phylogenetic backbone for prokaryotic systems.
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