M.Sc. (Semester II) (CBCS) Examination, 2017
ENVIRONMENTAL SCIENCE
ANALYTICAL TECHNIQUES AND INSTRUMENTATION
Day Date: Friday, 28-04-2017 Max. Marks: 70
Time: 10.30 AM to 01.00 PM
Instructions: Answer any five questions.
All questions carry equal marks.
Question 1 is compulsory.
Answer any two from Q.No.2, 4.
Answer any two from Q.No.5, 7.
Q.1 Select the answer among the following: 14
Difference in electrical potential between an electrode and a reference
electrode is measured by using
Electrical Conductivity Meter Potentiometer
pH Meter None of these
Turbidity in water is considered as types of perameter.
Biological Parameter Chemical Parameter
Physical Parameter None of these
In GC-MS the column provides a surface for the components in the
sample interacts is known as
Stationery phase Mobile phase
interactive phase None of these
Geiger Muller counters are used to detect radioactive emission, most
commonly and rays.
Alfa and Beta rays Beta and Gamma rays
Alfa and Gamma rays All of these
Detection of metals and non-metals at ppq range is detected by using

ICPS AAS
GC-MS UV spectrophotometer
Piece of light microscope that gather and focuses light on specimen being
viewed is known as
Objective Lance Eyepiece Lens
Condenser Lens Coarse Focus
In reverse phase HPLC
A hydrophobic stationary phase is combined with a polar mobile phase
A hydrophilic stationary phase is combined with a polar mobile phase
A hydrophilic stationary phase is combined with a non-polar mobile
phase
A hydrophobic stationary phase is combined with a non-polar mobile
phase.
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X-ray different fails to detect the presence of substances
Comprising less than 5 percent of a mixture
Containing a magnetic field
Comprising elements with two or more isotopes
Containing a high concentration of a carbon
method is used for the quantitative determination.
Turbidometric Method Titrimetric Method
Gravimetric Method None of these
10) system is used for the removal of particulate matter from
industrial exhaust streams.
Electrostatic precipitator Bag filters
Wet Scrubber All of these
11) Which of the following is not a step in atomic absorption spectroscopy?
Calibration curve is constructed
Atoms absorb light
Particles are absorbed onto a stationery phase
A solution is vaporized
12) Which of the following is a cytoplasmic Stain
Eosin Crystal Violet Carmine Orcein
13) What is shielding in NMR
Using a curved piece of metal to block an opponent's attack.
Putting metal around an Rf source.
Magnetic moment is blocks the full induced magnetic field from
surrounding nuclei.
Blocking parts of a molecule from Rf radiation
14) Electrostatic Precipitators are fully functioned in following
industry
Dairy Industry Pharmaceutical Industry
Thermal Power Plants Tanneries
Q.2 Define conductance of solution. Explain calibration of conductivity meter and
add a note on factors affecting conductance of solution.
14
Q.3 Explain with help of schematic diagram the operations of a double beam UV
spectrophotometer. Elaborate its advantages over single beam
spectrophotometer.
14
Q.4 What is Mass Spectroscopy? Describe role and different types of mass
spectrometer detectors.
14
Q.5 Write short notes on the following: 14
Gel Electrophoresis
Working of Flame Photometer
Q.6 Write a brief with suitable illustration on the following: 14
Role of RFLPs in DNA Fingerprinting
Need of reference electrode in pH meter
Q.7 Write an account on the following: 14
Working of Light Microscope
Turbidity Meter