M.Sc.(Genetic)(Semester IV) (CBCS) Examination, 2017
GENETIC ENGINEERING
Day Date: Wednesday, 19-04-2017 Marks: 70
Time: 2.30 PM to 5.00 PM
Instructions: Section-I is compulsory.
From Section-II attempt any four.
All questions carry equal marks.
Figures to right indicate full marks.
SECTION I
Q.1 Rewrite the following sentences by using correct alternative: 07
Which of the following is not required for a PCR reaction.
Dideoxy-dNTPS Primer
Template DNA A thermostable DNA polymerase
The vectors commonly used for sequencing of human genome

Yeast artificial chromosome Plasmid
CMV vectors M13 vectors
The variation in the restriction DNA fragment lengths between
individuals of species is called as
AFLP RAPD RFLP SSR
Tag polymerase is used in PCR because of its
low thermal stability high thermal stability
high speed slow speed
The virus commonly used to infect cell culture for the production
Of interferon is
corona virus Sendai virus
small pox virus Polio virus
The uptake of plasmid DNA into bacterial cell is facilited in the
presence of
Calcium Chloride Crystal bromide
crystal violet Fulgen
Guanine specific cleavage in Maxum-Gilbert method is done by
using
Formic acid Hydrazine
Dimethyl Sulphate Diethyl Phosphate
Page 2 of 2
Answer the following terms. 07
Define plasmid
Electroporation
What are restriction enzymes?
What is Cloning?
What is PCR?
DNA probes
What is Genetic engineering?
SECTION II
Attempt any four:
Q.2 Describe in deatail different steps involved in PCR reaction? 14
Q.3 What is gene transfer? Describe any two method of gene transfer. 14
Q.4 Describe Maxim's and Gilbert's method of DNA sequencing. 14
Q.5 Answer any two of the following: 14
Write note on RFLP its application.
Discuss Agrobacterium is considered as Natural Genetic engineer of
plant.
Discuss production of recombinant insulin
Q.6 Answer any two of the following: 14
Write account on restriction endonuclease
Describe in short YAC
Write note on herbicide resistance plant