P.G. DIPLOMA IN STRUCTURAL
PHARMACOGENOMICS EXAMINATION,
DECEMBER 2017
First Semester
Bioinformatics
Lab II STRUCTURAL BIOLOGY (THEORY)
(CBCS 2013 onwards)
Time 3 Hours Maximum 75 Marks
Part A (10 x 2 20)
Answer all questions.
1. Define cDNA.
2. What is cloning?
3. What are the protein structure determination methods?
4. What is X-Ray diffraction?
5. What are the types of plasmids?
6. What is phase problem?
7. What is structure refinement?
8. Define SNPs.
9. Define chromatography.
10. How mutation occurs?
Sub. Code
510106
RW-1395
2
Ws8
Part B x 5 25)
Answer all questions.
All questions carry equal marks.
11. Write about plasmid isolation.
Or
Explain briefly about cloning.
12. Explain Microbatch crystallization method.
Or
Explain hanging drop method.
13. Discuss briefly about NMR spectroscopy.
Or
Explain about protein structure refinement.
14. How can we determine random mutations?
Or
Explain about X-Ray diffraction data collection
methods.
15. Explain about Recombinant DNA technology.
Or
Describe about Gel filtration method.
RW-1395
3
Ws8
Part C x 10 30)
Answer any three of the following.
16. Explain in detail about cDNA library construction.
17. Explain in detail about protein purification.
18. Explain in detail about X-Ray crystallography method.
19. Explain about various types of mutations.
20. Give an overview of protein expression.